Academic Journal
In Vitro Activity of Pentamidine Alone and in Combination with Antibiotics against Multidrug-Resistant Clinical Pseudomonas aeruginosa Strains
| العنوان: | In Vitro Activity of Pentamidine Alone and in Combination with Antibiotics against Multidrug-Resistant Clinical Pseudomonas aeruginosa Strains |
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| المؤلفون: | Herrera-Espejo, Soraya, Cebrero-Cangueiro, Tania, Labrador-Herrera, Gema, Pachón, Jerónimo, Pachón-Ibáñez, M. E., Álvarez-Marín, Rocío |
| المساهمون: | Instituto de Salud Carlos III, Ministerio de Economía, Industria y Competitividad (España), Universidad de Sevilla, Junta de Andalucía, Ministerio de Ciencia, Innovación y Universidades (España), Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica, European Commission |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2020 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Pseudomonas aeruginosa, Multidrug-resistance, Antibiotics, Pentamidine, In vitro activity |
| الوصف: | © 2020 by the authors. ; Multidrug-resistant (MDR) Pseudomonas aeruginosa is a public health problem causing both community and hospital-acquired infections, and thus the development of new therapies for these infections is critical. The objective of this study was to analyze in vitro the activity of pentamidine as adjuvant in combinations to antibiotics against seven clinical P. aeruginosa strains. The Minimum Inhibitory Concentration (MIC) was determined following standard protocols, and the results were interpreted according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints; however, the gentamicin activity was interpreted according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. The bactericidal in vitro activity was studied at 1×MIC concentrations by time–kill curves, and also performed in three selected strains at 1/2×MIC of pentamidine. All studies were performed in triplicate. The pentamidine MIC range was 400–1600 μg/mL. Four of the strains were MDR, and the other three were resistant to two antibiotic families. The combinations of pentamidine at 1×MIC showed synergistic activity against all the tested strains, except for pentamidine plus colistin. Pentamidine plus imipenem and meropenem were the combinations that showed synergistic activity against the most strains. At 1/2×MIC, pentamidine plus antibiotics were synergistic with all three analyzed strains. In summary, pentamidine in combination with antibiotics showed in vitro synergy against multidrug-resistant P. aeruginosa clinical strains, which suggests its possible use as adjuvant to antibiotics for the therapy of infections from MDR P. aeruginosa. ; This study was supported by the Instituto de Salud Carlos III, Proyectos de Investigación en Salud (PI18-01842), Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/0009)—co-financed by European ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 2079-6382 |
| Relation: | Publisher's version; http://dx.doi.org/10.3390/antibiotics9120885; Sí; Antibiotics 9(12): 885 (2020); http://hdl.handle.net/10261/237426; http://dx.doi.org/10.13039/501100004587; http://dx.doi.org/10.13039/501100000780; http://dx.doi.org/10.13039/100009042; http://dx.doi.org/10.13039/501100010198; http://dx.doi.org/10.13039/501100011011 |
| DOI: | 10.3390/antibiotics9120885 |
| DOI: | 10.13039/501100004587 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/100009042 |
| DOI: | 10.13039/501100010198 |
| DOI: | 10.13039/501100011011 |
| الاتاحة: | http://hdl.handle.net/10261/237426 https://doi.org/10.3390/antibiotics9120885 https://doi.org/10.13039/501100004587 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/100009042 https://doi.org/10.13039/501100010198 https://doi.org/10.13039/501100011011 |
| Rights: | open |
| رقم الانضمام: | edsbas.989B30D7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20796382 |
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| DOI: | 10.3390/antibiotics9120885 |