Academic Journal
Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers
| العنوان: | Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers |
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| المؤلفون: | Harper, A., Brannigan, J.A., Buck, M., Hewitt, L., Moore, M.H., Schneider, B., Lewis, R.J. |
| سنة النشر: | 1998 |
| المجموعة: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| الوصف: | The X-ray crystal structure of d(TGCGCA)(2) has been determined at 120 K to a resolution of 1.3 Angstrom. Hexamer duplexes, in the Z-DNA conformation, pack in an arrangement similar to the 'pure spermine form' [Egli et al. (1991). Biochemistry, 30, 11388-11402] but with significantly different cell dimensions. The phosphate backbone exists in two equally populated discrete conformations at one nucleotide step, around phosphate 11. The structure contains two ordered cobalt hexammine molecules which have roles in stabilization of both the Z-DNA conformation of the duplex and in crystal packing. A comparison of d(TGCGCA)(2) with other Z-DNA hexamer structures available in the Nucleic Acid Database illustrates the elusive nature of crystal packing. A review of the interactions with the metal cations Na+, Mg2+ and Co3+ reveals a relatively small proportion of phosphate binding and that close contacts between metal ions are common. A prediction of the water structure is compared with the observed pattern in the reported structure. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://eprints.whiterose.ac.uk/452/1/branniganja10.pdf; Harper, A., Brannigan, J.A., Buck, M. et al. (4 more authors) (1998) Structure of d(TGCGCA)(2) and a comparison with other DNA Hexamers. Acta Crystallographica. Section D, Biological Crystallography. pp. 1273-1284. ISSN 1399-0047 |
| DOI: | 10.1107/S0907444998002807 |
| الاتاحة: | https://eprints.whiterose.ac.uk/452/ https://eprints.whiterose.ac.uk/452/1/branniganja10.pdf https://doi.org/10.1107/S0907444998002807 |
| رقم الانضمام: | edsbas.986519C2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1107/S0907444998002807 |
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