Academic Journal
RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growth
| العنوان: | RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growth |
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| المؤلفون: | Álvarez-Lindo, Noemí, Baleriola, Jimena, Ríos, Vivian de los, Suárez, Teresa, De la Rosa, Enrique J. |
| المساهمون: | Ministerio de Economía y Competitividad (España), Álvarez-Lindo, Noemí, Baleriola, Jimena, de los Ríos, Vivian, De la Rosa, Enrique J. |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Recombination-activating gene-1, Strand break repair, L1 retrotransposition, Neuronal migration, DNA-damage, Light-chain, Mice, Mutations, Choice, Expression |
| الوصف: | 12 p.-5 fig.-1 tab. ; DNA double-strand breaks (DSBs), selectively visualized as gamma-H2AX(+) foci, occur during the development of the central nervous system, including the retina, although their origin and biological significance are poorly understood. Mutant mice with DSB repair mechanism defects exhibit increased numbers of gamma-H2AX(+) foci, increased cell death during neural development, and alterations in axonogenesis in the embryonic retina. The aim of this study was to identify putative sources of DSBs. One of the identified DSBs sources is LINE-1 retrotransposition. While we did not detect changes in LINE-1 DNA content during the early period of cell death associated with retinal neurogenesis, retinal development was altered in mice lacking RAG-2, a component of the RAG-1,2-complex responsible for initiating somatic recombination in lymphocytes. Although gamma-H2AX(+) foci were less abundant in the rag2(-/-) mouse retina, retinal ganglion cell death was increased and axonal growth and navigation were impaired in the RAG-2 deficient mice, a phenotype shared with mutant mice with defective DNA repair mechanisms. These findings demonstrate that RAG-2 is necessary for proper retinal development, and suggest that both DSB generation and repair are genuine processes intrinsic to neural development. ; This work was supported by the Ministerio de Economía y Competitividad, Spain (Grants SAF2013-41059-R and SAF2016-75681R to EJdlR). ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-41059-R; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-75681-R; Publisher's version; https://doi.org/10.1038/s41598-019-54873-w; Sí; Scientific Reports 9(1)18486 (2019); http://hdl.handle.net/10261/197241; http://dx.doi.org/10.13039/501100003329 |
| DOI: | 10.1038/s41598-019-54873-w |
| DOI: | 10.13039/501100003329 |
| الاتاحة: | http://hdl.handle.net/10261/197241 https://doi.org/10.1038/s41598-019-54873-w https://doi.org/10.13039/501100003329 |
| Rights: | open |
| رقم الانضمام: | edsbas.980C144F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-019-54873-w |