LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

التفاصيل البيبلوغرافية
العنوان:	LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways
المؤلفون:	Kshirsagar, Aditya, Doroshev, Svetlana Maslov, Gorelik, Anna, Olender, Tsviya, Sapir, Tamar, Tsuboi, Daisuke, Rosenhek-Goldian, Irit, Malitsky, Sergey, Itkin, Maxim, Argoetti, Amir, Mandel-Gutfreund, Yael, Cohen, Sidney R., Hanna, Jacob H., Ulitsky, Igor, Kaibuchi, Kozo, Reiner, Orly
المصدر:	Nature Communications ; volume 14, issue 1 ; ISSN 2041-1723
بيانات النشر:	Springer Science and Business Media LLC
سنة النشر:	2023
الوصف:	Lissencephaly-1 ( LIS1 ) is associated with neurodevelopmental diseases and is known to regulate the molecular motor cytoplasmic dynein activity. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it governs the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overexpression partially rescued the extracellular matrix (ECM) expression and mechanosensitive genes conferring stiffness to Argonaute null mESCs. Collectively, our data transforms the current perspective on the roles of LIS1 in post-transcriptional regulation underlying development and mechanosensitive processes.
نوع الوثيقة:	article in journal/newspaper
اللغة:	English
DOI:	10.1038/s41467-023-38797-8
الاتاحة:	http://dx.doi.org/10.1038/s41467-023-38797-8 https://www.nature.com/articles/s41467-023-38797-8.pdf https://www.nature.com/articles/s41467-023-38797-8
Rights:	https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0
رقم الانضمام:	edsbas.978BF70C
قاعدة البيانات:	BASE

View record in BASE

الوصف
DOI:	10.1038/s41467-023-38797-8