Academic Journal
Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon
| العنوان: | Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon |
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| المؤلفون: | Klinkert, Birgit, Elles, Ingolf, Nickelsen, Jörg |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2006 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Articles |
| الوصف: | Translation initiation represents a key step during regulation of gene expression in chloroplasts. Here, we report on the identification and characterization of three suppressor point mutations which overcome a translational defect caused by the deletion of a U-rich element in the 5′-untranslated region (5′-UTR) of the psbD mRNA in the green alga Chlamydomonas reinhardtii . All three suppressors affect a secondary RNA structure encompassing the psbD AUG initiation codon within a double-stranded region as judged by the analysis of site-directed chloroplast mutants as well as in vitro RNA mapping experiments using RNase H. In conclusion, the data suggest that these new element serves as a negative regulator which mediates a rapid shut-down of D2 synthesis. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://nar.oxfordjournals.org/cgi/content/short/34/1/386; http://dx.doi.org/10.1093/nar/gkj433 |
| DOI: | 10.1093/nar/gkj433 |
| الاتاحة: | http://nar.oxfordjournals.org/cgi/content/short/34/1/386 https://doi.org/10.1093/nar/gkj433 |
| Rights: | Copyright (C) 2006, Oxford University Press |
| رقم الانضمام: | edsbas.972068A3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkj433 |
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