Academic Journal
Enrichment and isolation of Flavobacterium strains with tolerance to high concentrations of cesium ion
| العنوان: | Enrichment and isolation of Flavobacterium strains with tolerance to high concentrations of cesium ion |
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| المؤلفون: | Kato, Souichiro, Goya, Eri, Tanaka, Michiko, Kitagawa, Wataru, Kikuchi, Yoshitomo, Asano, Kozo, Kamagata, Yoichi |
| المصدر: | Scientific Reports ; volume 6, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2016 |
| الوصف: | Interest in the interaction of microorganisms with cesium ions (Cs + ) has arisen, especially in terms of their potent ability for radiocesium bioaccumulation and their important roles in biogeochemical cycling. Although high concentrations of Cs + display toxic effects on microorganisms, there have been only limited reports for Cs + -tolerant microorganisms. Here we report enrichment and isolation of Cs + -tolerant microorganisms from soil microbiota. Microbial community analysis revealed that bacteria within the phylum Bacteroidetes , especially Flavobacterium spp., dominated in enrichment cultures in the medium supplemented with 50 or 200 mM Cs + , while Gammaproteobacteria was dominant in the control enrichment cultures (in the presence of 50 and 200 mM K + instead of Cs + ). The dominant Flavobacterium sp. was successfully isolated from the enrichment culture and was closely related to Flavobacterium chungbukense with 99.5% identity. Growth experiments clearly demonstrated that the isolate has significantly higher tolerance to Cs + compared to its close relatives, suggesting the Cs + -tolerance is a specific trait of this strain, but not a universal trait in the genus Flavobacterium . Measurement of intracellular K + and Cs + concentrations of the Cs + -tolerant isolate and its close relatives suggested that the ability to maintain low intracellular Cs + concentration confers the tolerance against high concentrations of external Cs + . |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/srep20041 |
| الاتاحة: | http://dx.doi.org/10.1038/srep20041 https://www.nature.com/articles/srep20041 https://www.nature.com/articles/srep20041.pdf |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.96D60089 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/srep20041 |
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