Academic Journal
Differential expression of a prophage-encoded glycocin and its immunity protein suggests a mutualistic strategy of a phage and its host
| العنوان: | Differential expression of a prophage-encoded glycocin and its immunity protein suggests a mutualistic strategy of a phage and its host |
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| المؤلفون: | Denham, Emma L, Piersma, Sjouke, Rinket, Marleen, Reilman, Ewoud, de Goffau, Marcus C, van Dijl, Jan Maarten |
| المصدر: | Denham , E L , Piersma , S , Rinket , M , Reilman , E , de Goffau , M C & van Dijl , J M 2019 , ' Differential expression of a prophage-encoded glycocin and its immunity protein suggests a mutualistic strategy of a phage and its host ' , Scientific Reports , vol. 9 , no. 1 , 2845 . https://doi.org/10.1038/s41598-019-39169-3 |
| سنة النشر: | 2019 |
| المجموعة: | University of Groningen research database |
| مصطلحات موضوعية: | Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacteriocins/genetics, Escherichia coli, Gene Expression Regulation, Bacterial, Viral, Glycopeptides/genetics, Prophages/genetics, Symbiosis, Transcription Factors/metabolism, Transcriptional Activation, Viral Proteins/genetics |
| الوصف: | Sublancin 168 is a highly potent and stable antimicrobial peptide secreted by the Gram-positive bacterium Bacillus subtilis. Production of sublancin gives B. subtilis a major competitive growth advantage over a range of other bacteria thriving in the same ecological niches, the soil and plant rhizosphere. B. subtilis protects itself against sublancin by producing the cognate immunity protein SunI. Previous studies have shown that both the sunA gene for sublancin and the sunI immunity gene are encoded by the prophage SP beta. The sunA gene is under control of several transcriptional regulators. Here we describe the mechanisms by which sunA is heterogeneously expressed within a population, while the sunI gene encoding the immunity protein is homogeneously expressed. The key determinants in heterogeneous sunA expression are the transcriptional regulators Spo0A, AbrB and Rok. Interestingly, these regulators have only a minor influence on sunI expression and they have no effect on the homogeneous expression of sunI within a population of growing cells. Altogether, our findings imply that the homogeneous expression of sunI allows even cells that are not producing sublancin to protect themselves at all times from the active sublancin produced at high levels by their isogenic neighbors. This suggests a mutualistic evolutionary strategy entertained by the SP beta prophage and its Bacillus host, ensuring both stable prophage maintenance and a maximal competitive advantage for the host at minimal costs. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://research.rug.nl/en/publications/7e4dcd24-c4fa-4605-a8a5-662c3210694c |
| DOI: | 10.1038/s41598-019-39169-3 |
| الاتاحة: | https://hdl.handle.net/11370/7e4dcd24-c4fa-4605-a8a5-662c3210694c https://research.rug.nl/en/publications/7e4dcd24-c4fa-4605-a8a5-662c3210694c https://doi.org/10.1038/s41598-019-39169-3 https://pure.rug.nl/ws/files/78114586/Differential_expression_of_a_prophage_encoded_glycocin_and_its_immunity_protein_suggests_a_mutualistic_strategy_of_a_phage_and_its_host.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.96653A3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-019-39169-3 |
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