Academic Journal
Use of Bacterial Toxin–Antitoxin Systems as Biotechnological Tools in Plants
| العنوان: | Use of Bacterial Toxin–Antitoxin Systems as Biotechnological Tools in Plants |
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| المؤلفون: | Bernardo Rodamilans, Xiaofei Cheng, Carmen Simón-Mateo, Juan Antonio García |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 19, p 10449 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | toxin, antitoxin, protease, NIa, potyvirus, antiviral, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Toxin–antitoxin (TA) systems in bacteria are key regulators of the cell cycle and can activate a death response under stress conditions. Like other bacterial elements, TA modules have been widely exploited for biotechnological purposes in diverse applications, such as molecular cloning and anti-cancer therapies. However, their use in plants has been limited, leaving room for the development of new approaches. In this study, we examined two TA systems previously tested in plants, MazEF and YefM-YoeB, and identified interesting differences between them, likely related to their modes of action. We engineered modifications to these specific modules to transform them into molecular switches that can be activated by a protease, inducing necrosis in the plant cells where they are expressed. Finally, we demonstrated the antiviral potential of the modified TA modules by using, as a proof-of-concept, the potyvirus plum pox virus as an activator of the death phenotype. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://www.mdpi.com/1422-0067/25/19/10449; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/59b85390695a4465968eeacb89559bf4 |
| DOI: | 10.3390/ijms251910449 |
| الاتاحة: | https://doi.org/10.3390/ijms251910449 https://doaj.org/article/59b85390695a4465968eeacb89559bf4 |
| رقم الانضمام: | edsbas.9643F38D |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms251910449 |
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