Academic Journal
Venomics of the Central American Lyre snake Trimorphodon quadruplex (Colubridae: Smith, 1941) from Costa Rica
| العنوان: | Venomics of the Central American Lyre snake Trimorphodon quadruplex (Colubridae: Smith, 1941) from Costa Rica |
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| المؤلفون: | Mackessy, Stephen P., Bryan, Wendy, Smith, Cara F., Lopez, Keira, Fernández Ulate, Julián, Bonilla Murillo, Fabián, Camacho Umaña, Erika, Sasa Marín, Mahmood, Lomonte, Bruno |
| المصدر: | Journal of Proteomics (2020) 220, 103778 |
| سنة النشر: | 2020 |
| المجموعة: | Universidad de Costa Rica: Repositorio Kérwá |
| مصطلحات موضوعية: | proteomics, snake venom, Colubridae |
| الوصف: | Rear-fanged colubrid snakes include hundreds of species globally that possess a Duvernoy's venom gland and often one-several enlarged rear maxillary teeth. We investigated the venom proteome of the Central American Lyre Snake (Trimorphodon quadruplex), a moderate-sized rear-fanged colubrid snake and the southernmost Trimorphodon, using a bottom-up proteomic approach coupled with enzyme and inhibitor assays, cytotoxicity assays and lethal toxicity assays. Several enzymes uncommonly observed in colubrid venoms were purified and characterized further. Trimorphodon quadruplex has a rather low complexity venome, typical of many rear-fanged snakes, but its venom contains L-amino acid oxidase, phospholipase A2, and a dimeric 3FTx, and 3FTxs dominate the proteome. Its PLA2 is catalytically quite active, but it lacks myotoxicity or acute toxicity; LAAO exhibits conserved structure and appears to be highly labile. Several P-III metalloproteinases are present and hydrolyze azocasein and the α-subunit of fibrinogen but lack hemorrhagic activity. Trimorphodon quadruplex produces venom and retains constriction, utilizing both chemically-mediated and mechanical feeding modes. ; UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-1-874391-92-0 1-874391-92-0 |
| تدمد: | 1874-3919 |
| Relation: | https://www.sciencedirect.com/science/article/abs/pii/S1874391920301469?via%3Dihub; https://hdl.handle.net/10669/82966 |
| DOI: | 10.1016/j.jprot.2020.103778 |
| الاتاحة: | https://hdl.handle.net/10669/82966 https://www.sciencedirect.com/science/article/abs/pii/S1874391920301469?via%3Dihub https://doi.org/10.1016/j.jprot.2020.103778 |
| Rights: | acceso abierto |
| رقم الانضمام: | edsbas.96412CD7 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781874391920 1874391920 |
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| تدمد: | 18743919 |
| DOI: | 10.1016/j.jprot.2020.103778 |