التفاصيل البيبلوغرافية
| العنوان: |
Role of CCR7 in skin infection. |
| المؤلفون: |
E. Yaneth Osorio (9226059), Ashanti Uscanga-Palomeque (13186353), Grace E. Patterson (14381117), Erika Cordova (12754823), Bruno L. Travi (9226065), Lynn Soong (175074), Peter C. Melby (9226062) |
| سنة النشر: |
2023 |
| مصطلحات موضوعية: |
Microbiology, Molecular Biology, Ecology, Immunology, Developmental Biology, Infectious Diseases, Virology, Computational Biology, sand fly vector, found enhanced capture, div >< p, subclinical latent infection, dtr transgenic mice, early parasite dissemination, people developing vl, leishmania donovani <, infected people develop, related parasite dissemination, infected inflammatory monocytes, parasite dissemination, donovani <, malnourished mice, inflammatory monocytes, active vl, related trafficking, l <, visceral organs, visceral leishmaniasis, skin infection, results show |
| الوصف: |
A . CCR7 in BM monocytes transfected with control (scrambled) siRNA or siRNA targeting CCR7. Determined by qRT-PCR and expressed as fold-change over control(Left) or percentage of CCR7+ monocytes by flow cytometry (Right). *** p <0.0001 (Unpaired t-test). 4 replicates per group. B. BM derived monocytes transfected with CCR7-targeting siRNA or control siRNA, labeled and transferred ID to malnourished mice at the same time as infection ID with cell trace-labeled L . donovani . Percent of transferred monocytes in the skin (Left panel) and intensity of infection of transferred monocytes in the skin (right panel) determined by flow cytometry at 48h p.i. N = 10 mice per group. C. Proportion of Transferred monocytes (left panel) and intensity of the infection (right panel) in lymph node (LN) of malnourished mice recipients of monocytes transfected with CCR7-targeting siRNA or control siRNA. N = 9–10 per group. D. Percent of infected monocytes found in the lymph node (LN) of malnourished mice recipients of WT or CCR7KO donor monocytes 48 hr after of intradermal transfer and ID infection with mcherry- L . donovani . Donor cells identified with a Far-red cell trace and flow cytometry. Infected monocytes discriminated by the expression of Ly6C (Ly6C int/hi , inflammatory monocytes; Ly6C low/- , resident non-inflammatory monocytes). * p = 0.057 (Mann-Whitney test). N = 4 mice per group. (TIF) |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Role_of_CCR7_in_skin_infection_/21867515 |
| DOI: |
10.1371/journal.pntd.0011040.s011 |
| الاتاحة: |
https://doi.org/10.1371/journal.pntd.0011040.s011 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.960D61E0 |
| قاعدة البيانات: |
BASE |