Academic Journal
Utilizing transcriptomics and proteomics to unravel key genes and proteins of Oryza sativa seedlings mediated by selenium in response to cadmium stress
| العنوان: | Utilizing transcriptomics and proteomics to unravel key genes and proteins of Oryza sativa seedlings mediated by selenium in response to cadmium stress |
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| المؤلفون: | Zhu, Sixi, Sun, Suxia, Zhao, Wei, Yang, Xiuqin, Mao, Huan, Sheng, Luying, Chen, Zhongbing |
| المساهمون: | the 2024 Guizhou Basic Research Plan (Natural Science) project, China, the National Natural Science Foundation of China |
| المصدر: | BMC Plant Biology ; volume 24, issue 1 ; ISSN 1471-2229 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2024 |
| الوصف: | Background Cadmium (Cd) pollution has declined crop yields and quality. Selenium (Se) is a beneficial mineral element that protects plants from oxidative damage, thereby improving crop tolerance to heavy metals. The molecular mechanism of Se-induced Cd tolerance in rice (Oryza sativa) is not yet understood. This study aimed to elucidate the beneficial mechanism of Se (1 mg/kg) in alleviating Cd toxicity in rice seedlings. Results Exogenous selenium addition significantly improved the toxic effect of cadmium stress on rice seedlings, increasing plant height and fresh weight by 20.53% and 34.48%, respectively, and increasing chlorophyll and carotenoid content by 16.68% and 15.26%, respectively. Moreover, the MDA, ·OH, and protein carbonyl levels induced by cadmium stress were reduced by 47.65%, 67.57%, and 56.43%, respectively. Cell wall metabolism, energy cycling, and enzymatic and non-enzymatic antioxidant systems in rice seedlings were significantly enhanced. Transcriptome analysis showed that the expressions of key functional genes psbQ, psbO, psaG, psaD, atpG, and PetH were significantly up-regulated under low-concentration Se treatment, which enhanced the energy metabolism process of photosystem I and photosystem II in rice seedlings. At the same time, the up-regulation of LHCA, LHCB family, and C4H1, PRX, and atp6 functional genes improved the ability of photon capture and heavy metal ion binding in plants. Combined with proteome analysis, the expression of functional proteins OsGSTF1, OsGSTU11, OsG6PDH4, OsDHAB1, CP29, and CabE was significantly up-regulated under Se, which enhanced photosynthesis and anti-oxidative stress mechanism in rice seedlings. At the same time, it regulates the plant hormone signal transduction pathway. It up-regulates the expression response process of IAA, ABA, and JAZ to activate the synergistic effect between each cell rapidly and jointly maintain the homeostasis balance. Conclusion Our results revealed the regulation process of Se-mediated critical metabolic pathways, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/s12870-024-05076-7 |
| DOI: | 10.1186/s12870-024-05076-7.pdf |
| DOI: | 10.1186/s12870-024-05076-7/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/s12870-024-05076-7 https://link.springer.com/content/pdf/10.1186/s12870-024-05076-7.pdf https://link.springer.com/article/10.1186/s12870-024-05076-7/fulltext.html |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.95CDFD85 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12870-024-05076-7 |
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