التفاصيل البيبلوغرافية
| العنوان: |
DataSheet_1_“Cerberus” T Cells: A Glucocorticoid-Resistant, Multi-Pathogen Specific T Cell Product to Fight Infections in Severely Immunocompromised Patients.docx |
| المؤلفون: |
Kiriakos Koukoulias (9997373), Penelope-Georgia Papayanni (9997376), Aphrodite Georgakopoulou (9997379), Maria Alvanou (9997382), Stamatia Laidou (9997385), Anastasios Kouimtzidis (9997388), Chrysoula Pantazi (9997391), Glykeria Gkoliou (9997394), Timoleon-Achilleas Vyzantiadis (9997397), Alexandros Spyridonidis (4050133), Antonios Makris (3523544), Anastasia Chatzidimitriou (9997400), Nikoletta Psatha (6998765), Achilles Anagnostopoulos (5767109), Evangelia Yannaki (9997403), Anastasia Papadopoulou (9997406) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, cytomegalovirus, Epstein-Barr virus, adenovirus, BK virus, Aspergillus fumigatus, T cell therapy |
| الوصف: |
Adoptive immunotherapy (AI) with pathogen-specific T cells is a promising alternative to pharmacotherapy for the treatment of opportunistic infections after allogeneic hematopoietic cell transplantation or solid organ transplantation. However, clinical implementation of AI is limited to patients not receiving high-dose steroids, a prerequisite for optimal T-cell function, practically excluding the most susceptible to infections patients from the benefits of AI. To address this issue, we here rapidly generated, clinical doses of a steroid-resistant T-cell product, simultaneously targeting four viruses (adenovirus, cytomegalovirus, Epstein Barr virus, and BK virus) and the fungus Aspergillus fumigatus, by genetic disruption of the glucocorticoid receptor (GR) gene using CRISPR/CAS9 ribonucleoprotein delivery. The product, “Cerberus” T cells (Cb-STs), was called after the monstrous three-headed dog of Greek mythology, due to its triple potential; specificity against viruses, specificity against fungi and resistance to glucocorticoids. Following efficient on-target GR disruption and minimal off-target editing, the generated Cb-STs maintained the characteristics of pentavalent-STs, their unedited counterparts, including polyclonality, memory immunophenotype, specificity, and cytotoxicity while they presented functional resistance to dexamethasone. Cb-STs may become a powerful, one-time treatment for severely immunosuppressed patients under glucocorticoids who suffer from multiple, life-threatening infections post-transplant, and for whom therapeutic choices are limited. |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/DataSheet_1_Cerberus_T_Cells_A_Glucocorticoid-Resistant_Multi-Pathogen_Specific_T_Cell_Product_to_Fight_Infections_in_Severely_Immunocompromised_Patients_docx/13601915 |
| DOI: |
10.3389/fimmu.2020.608701.s001 |
| الاتاحة: |
https://doi.org/10.3389/fimmu.2020.608701.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.95BDE2E2 |
| قاعدة البيانات: |
BASE |