Academic Journal
Conformational Effects of Serine Phosphorylation in Phospholamban Peptides
| العنوان: | Conformational Effects of Serine Phosphorylation in Phospholamban Peptides |
|---|---|
| المؤلفون: | Quirk, Philip G., Patchell, Valerie B., Colyer, John, Drago, Guido A., Gao, Yuan |
| المصدر: | European Journal of Biochemistry ; volume 236, issue 1, page 85-91 ; ISSN 0014-2956 1432-1033 |
| بيانات النشر: | Wiley |
| سنة النشر: | 1996 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | We have employed one‐ and two‐dimensional 1 H‐NMR spectroscopy to study the effects of serine phosphorylation on peptide conformations, using cardiac phospholamban as a model system. The nonphosphorylated phospholamban 1–20 peptide has few restraints on the conformations available to it in aqueous solution. Phosphorylation at Ser16 results in greater constraints being placed on the region encompassing Arg14–Thr17, particularly at neutral pH when the phosphate group is in the di‐anionic form. These conformational restrictions arise from specific interactions between the side‐chain of Arg14 and the phosphate group. While substitution of phosphothreonine at position 16 causes generally similar effects to phosphoserine, aspartic acid has little effect. The results are compared with phosphorylation effects in other systems, including cardiac troponin I. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/j.1432-1033.1996.00085.x |
| الاتاحة: | http://dx.doi.org/10.1111/j.1432-1033.1996.00085.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1996.00085.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1996.00085.x |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.95ACFE49 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/j.1432-1033.1996.00085.x |
|---|