Academic Journal
Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton
| العنوان: | Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton |
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| المؤلفون: | Barabas, Federico Martín, Masullo, Luciano Andrés, Bordenave, Martín Diego, Giusti, Sebastian Alejandro, Unsain, Nicolas, Refojo, Damian, Caceres, Alfredo Oscar, Stefani, Fernando Daniel |
| بيانات النشر: | Nature Publishing Group |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | PROTEIN SELF-ASSEMBLY, SUPER-RESOLUTION, NEURON, CYTOSKELETON, https://purl.org/becyt/ford/1.6, https://purl.org/becyt/ford/1 |
| الوصف: | Fluorescence nanoscopy imaging permits the observation of periodic supramolecular protein structures in their natural environment, as well as the unveiling of previously unknown protein periodic structures. Deciphering the biological functions of such protein nanostructures requires systematic and quantitative analysis of large number of images under different experimental conditions and specific stimuli. Here we present a method and an open source software for the automated quantification of protein periodic structures in super-resolved images. Its performance is demonstrated by analyzing the abundance and regularity of the spectrin membrane-associated periodic skeleton (MPS) in hippocampal neurons of 2 to 40 days in vitro, imaged by STED and STORM nanoscopy. The automated analysis reveals that both the abundance and the regularity of the MPS increase over time and reach maximum plateau values after 14 DIV. A detailed analysis of the distributions of correlation coefficients provides indication of dynamical assembly and disassembly of the MPS. ; Fil: Barabas, Federico Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina ; Fil: Masullo, Luciano Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina ; Fil: Bordenave, Martín Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Centro de Investigaciones en Bionanociencias ; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina ; Fil: Giusti, Sebastian Alejandro. Consejo ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0068-1261 2045-2322 |
| Relation: | info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-017-16280-x; http://hdl.handle.net/11336/54317; Barabas, Federico Martín; Masullo, Luciano Andrés; Bordenave, Martín Diego; Giusti, Sebastian Alejandro; Unsain, Nicolas; et al.; Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: Abundance and regularity of neuronal spectrin membrane-associated skeleton; Nature Publishing Group; Scientific Reports; 7; 1; 12-2017; 1-10; CONICET Digital; CONICET |
| الاتاحة: | http://hdl.handle.net/11336/54317 |
| Rights: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/2.5/ar/ |
| رقم الانضمام: | edsbas.957F0B0 |
| قاعدة البيانات: | BASE |
| تدمد: | 00681261 20452322 |
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