Academic Journal
Beneficial effects of adipose-derived stromal vascular fraction on testicular injury caused by busulfan
| العنوان: | Beneficial effects of adipose-derived stromal vascular fraction on testicular injury caused by busulfan |
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| المؤلفون: | Hekimoglu, E. Rumeysa, Esrefoglu, Mukaddes, Karakaya Cimen, Fatma Bedia, Elibol, Birsen, Dedeakayogullari, Huri, Pasin, Ozge |
| بيانات النشر: | Taylor & Francis Ltd |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Busulfan, Stromal Vascular Fraction, Stem Cell, Infertility, Testicular Damage |
| الوصف: | The use of stem cells can attenuate testicular injury and promote sperm production. The adipose-derived stromal vascular fraction (SVF) has become an attractive cell source for cell-based therapies. In this study, we aimed to investigate the therapeutic efficacy of SVF on busulfan-induced testicular damage in rats. Twenty-four male rats were randomly divided into control, busulfan, SVF, and busulfan + SVF groups. Testicular damage was induced by intraperitoneal administration of busulfan (35 mg/kg). SVF obtained from human adipose tissue using Lipocube SVF (TM) was injected into rats 5 weeks after busulfan administration. At the end of the 8th week, rats were sacrificed, and histopathological, biochemical, and western blotting analyses were performed. No harmful effects of SVF on healthy testis tissue and sperm parameters were detected. SVF improved busulfan-induced oxidative stress in both testis tissue and serum. SVF injection to damaged testicular tissue resulted in increases in the healthy spermatozoon numbers and decreases in the abnormal tail numbers. Additionally, SVF increased bax/Bcl, DAZL, and TGF-beta 1 levels whereas decreased ATG5 and NF-kB levels. According to the results we obtained in this study, we suggest that SVF is beneficial in restoring damaged tissue by primarily being a multipotent cell source, by inhibiting oxidative stress and converting necrotic cell death to apoptotic cell death. In the future, clinical applications should bring higher benefits. Since SVF is the patient's own tissue, being harmless, it will offer an advantageous supportive treatment option for patients already weakened by cancer and anticancer therapy. ; Scientific Research Unit of Bezmialem Vakimath;f University (BAP) [20210825] ; The project was supported by the Scientific Research Unit of Bezmialem Vak & imath;f University (BAP No.: 20210825). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0148-0545 1525-6014 |
| Relation: | Drug and Chemical Toxicology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1080/01480545.2024.2324332; https://hdl.handle.net/20.500.12713/4834; WOS:001182638800001; 2-s2.0-85187447442; N/A; Q2 |
| DOI: | 10.1080/01480545.2024.2324332 |
| الاتاحة: | https://hdl.handle.net/20.500.12713/4834 https://doi.org/10.1080/01480545.2024.2324332 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9517827F |
| قاعدة البيانات: | BASE |
| تدمد: | 01480545 15256014 |
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| DOI: | 10.1080/01480545.2024.2324332 |