Academic Journal
Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer
| العنوان: | Paper-based (bio)sensor for label-free detection of 3-nitrotyrosine in human urine samples using molecular imprinted polymer |
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| المؤلفون: | Martins, Gabriela V., Marques, Ana C., Fortunato, Elvira, Sales, Maria Goreti Ferreira |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2020 |
| المجموعة: | Instituto Politécnico do Porto: Repositório Científico (RECIPP) |
| مصطلحات موضوعية: | 3-nitrotyrosine, Molecular imprinted polymer, Electrochemical (bio)sensor, Urine biomarker, Carbon-printed electrode |
| الوصف: | Over the last years, paper technology has been widely spread as a more affordable, sustainable and reliable support material to be incorporated in the design of point-of-care (POC) diagnostic devices. However, the single work employing a paper-based device for 3-nitrotyrosine (3-NT), a relevant biomarker for oxidative stress (OS) that is a major origin for many diseases, is incapable of reading successfully complex samples because every species that oxidizes before ~0.75 V will also contribute to the final response. Thus, the introduction of a selective element was made into this set-up by including a molecularly-imprinted polymer (MIP) tailored in-situ. Herein, a novel MIP for 3-NT was assembled directly on a paper platform, made conductive with carbon ink and suitable for an electrochemical transduction. The biomimetic material was produced by electropolymerization of phenol after optimizing several experimental parameters, such a scan-rate, number of cycles, range of potential applied, monomer and template concentrations. Under optimal conditions, the label-free sensor was able to respond to 3-NT from 500 nM to 1 mM, yielding a limit of detection of 22.3 nM. Finally, the applicability of the (bio)sensor was tested by performing calibration assays in human urine samples and a good performance was obtained in terms of sensitivity, selectivity and reproducibility. Overall, the attributes of the herein described sensing approach can be compared to a very limited number of other electrochemical devices, that are still using a conventional three electrode system, making this paper-sustained device the first electrochemical (bio)sensor with potential to become a portable and low-cost diagnostic tool for 3-NT. In general, the incorporation of molecular imprinting technology coupled to electrochemical transduction enabled the fabrication of suitable smart sensors for wide screening approaches. ; Fundação para a Ciência e Tecnologia (FCT) supported this work through FEDER funds from COMPETE 2020 Program and National ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-2-214-18041-9 2-214-18041-9 |
| Relation: | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-TEC%2F5400%2F2014/PT; POCI-01-0145-FEDER-016637; NORTE-01-0145-FEDER-024358; info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCTM%2F50025%2F2019/PT; info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F94159%2F2013/PT; info:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F115173%2F2016/PT; https://www.sciencedirect.com/science/article/pii/S2214180419302272?via%3Dihub; http://hdl.handle.net/10400.22/18588 |
| DOI: | 10.1016/j.sbsr.2020.100333 |
| الاتاحة: | http://hdl.handle.net/10400.22/18588 https://doi.org/10.1016/j.sbsr.2020.100333 |
| Rights: | openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.9505EAC2 |
| قاعدة البيانات: | BASE |
| ردمك: | 9782214180419 2214180419 |
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| DOI: | 10.1016/j.sbsr.2020.100333 |