Academic Journal
Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market
| العنوان: | Detection and molecular characterisation of methicillin-resistant Staphylococcus aureus isolated from raw meat in the retail market |
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| المؤلفون: | Tegegne, Henok, Ayalew, Koláčková, Ivana, Florianová, Martina, Gelbíčová, Tereza, Madec, Jean-Yves, Haenni, Marisa, Karpíšková, Renáta |
| المساهمون: | Veterinary Research Institute Brno (VRI), Université de Lyon, Unité Antibiorésistance et Virulence Bactériennes (AVB), Laboratoire de Lyon ANSES, Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université de Lyon-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), University of Veterinary and Pharmaceutical Sciences Brno (VFU), Ministry of Education, Youth and Sports (MEYS) CZ.1.05/2.1.00/19.0385 and MZE-RO0520 |
| المصدر: | ISSN: 2213-7165. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2021 |
| المجموعة: | Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| مصطلحات موضوعية: | MRSA, MLST, spa typing, Enterotoxin genes, Virulence genes, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| الوصف: | International audience ; Objectives: This study aimed to detect and characterise methicillin-resistant Staphylococcus aureus (MRSA) from retail meat in the Czech Republic. Methods: Isolates were identified by PCR detection of the S. aureus-specific fragment Sa442 and mecA gene. spa typing, MLST, detection of genes encoding staphylococcal enterotoxins, Panton-Valentine leukocidin (pvl), exfoliative toxins A and B (eta and etb), toxic shock syndrome toxin (tst) and staphylokinase (sak), detection of ϕSa3 prophage and antimicrobial susceptibility testing were performed. Results: Of 65 raw meat samples examined (poultry, beef, pork and rabbit), 23 (35.4%) were positive for MRSA. Twelve positive samples originated from poultry (12/33; 36.4%), while the remaining eleven came from pork (9/9; 100%) and pork/beef mixed minced meat (2/5; 40.0%). Eight spa types belonging to five different sequence types (STs) were identified. ST398 was the most frequent (28/36; 77.8%), presenting spa types t011, t034, t2576, t4132, t588 and t899. Other livestock-associated MRSA STs (ST9-t899, ST5-t002, ST692-t8646 or the newly described ST4034-t899) were also sporadically identified. In seven isolates (19.4%), one or more staphylococcal enterotoxin genes were detected, with sea, seg and sei prevailing. Three isolates from turkey [ST398-t899 (n = 2) and ST398-t011] harboured the sak gene, and the latter also harboured the sea gene. Seven isolates from poultry harboured the ϕSa3 prophage and were resistant to tetracycline. Conclusion: Specific kinds of meat appear to be a possible source of MRSA, although the risk to humans is hard to define. Therefore, surveillance of MRSA in meat as well as hygienic practices should be improved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | anses-03987610; https://anses.hal.science/anses-03987610; https://anses.hal.science/anses-03987610/document; https://anses.hal.science/anses-03987610/file/Tegegne_MRSAmeat_JGAR2021.pdf |
| DOI: | 10.1016/j.jgar.2021.06.012 |
| الاتاحة: | https://anses.hal.science/anses-03987610 https://anses.hal.science/anses-03987610/document https://anses.hal.science/anses-03987610/file/Tegegne_MRSAmeat_JGAR2021.pdf https://doi.org/10.1016/j.jgar.2021.06.012 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.94E5098C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jgar.2021.06.012 |
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