Academic Journal
Single high-dose intravenous injection of Wharton’s jelly-derived mesenchymal stem cell exerts protective effects in a rat model of metabolic syndrome
| العنوان: | Single high-dose intravenous injection of Wharton’s jelly-derived mesenchymal stem cell exerts protective effects in a rat model of metabolic syndrome |
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| المؤلفون: | Alvin Man Lung Chan, Angela Min Hwei Ng, Mohd Heikal Mohd Yunus, Ruszymah Hj Idrus, Jia Xian Law, Muhammad Dain Yazid, Kok-Yong Chin, Mohd Rafizul Mohd Yusof, See Nguan Ng, Benson Koh, Yogeswaran Lokanathan |
| المصدر: | Stem Cell Research & Therapy, Vol 15, Iss 1, Pp 1-19 (2024) |
| بيانات النشر: | BMC |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Cell therapy, Metabolic syndrome, Mesenchymal stem cell, Syndrome X Wharton’s jelly, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Background Metabolic syndrome (MetS) is a significant epidemiological problem worldwide. It is a pre-morbid, chronic and low-grade inflammatory disorder that precedes many chronic diseases. Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) could be used to treat MetS because they express high regenerative capacity, strong immunomodulatory properties and allogeneic biocompatibility. This study aims to investigate WJ-MSCs as a therapy against MetS in a rat model. Methods Twenty-four animals were fed with high-fat high-fructose (HFHF) diet ad libitum. After 16 weeks, the animals were randomised into treatment groups (n = 8/group) and received a single intravenous administration of vehicle, that is, 3 × 106 cells/kg or 10 × 106 cells/kg of WJ-MSCs. A healthy animal group (n = 6) fed with a normal diet received the same vehicle as the control (CTRL). All animals were periodically assessed (every 4 weeks) for physical measurements, serum biochemistry, glucose tolerance test, cardiovascular function test and whole-body composition. Post-euthanasia, organs were weighed and processed for histopathology. Serum was collected for C-reactive protein and inflammatory cytokine assay. Results The results between HFHF-treated groups and healthy or HFHF-CTRL did not achieve statistical significance (α = 0.05). The effects of WJ-MSCs were masked by the manifestation of different disease subclusters and continuous supplementation of HFHF diet. Based on secondary analysis, WJ-MSCs had major implications in improving cardiopulmonary morbidities. The lungs, liver and heart show significantly better histopathology in the WJ-MSC-treated groups than in the untreated CTRL group. The cells produced a dose-dependent effect (high dose lasted until week 8) in preventing further metabolic decay in MetS animals. Conclusions The establishment of safety and therapeutic proof-of-concept encourages further studies by improving the current therapeutic model. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1757-6512 |
| Relation: | https://doi.org/10.1186/s13287-024-03769-2; https://doaj.org/toc/1757-6512; https://doaj.org/article/d59ca73e73c74633bb4cbe6da82878d7 |
| DOI: | 10.1186/s13287-024-03769-2 |
| الاتاحة: | https://doi.org/10.1186/s13287-024-03769-2 https://doaj.org/article/d59ca73e73c74633bb4cbe6da82878d7 |
| رقم الانضمام: | edsbas.94DA151D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-024-03769-2 |