التفاصيل البيبلوغرافية
| العنوان: |
Peptidic Sulfhydryl for Interfacing Nanocrystals and Subsequent Sensing of SARS-CoV‑2 Protease |
| المؤلفون: |
Zhicheng Jin (2119099), Justin Yeung (8730369), Jiajing Zhou (702952), Yong Cheng (237211), Yi Li (1144), Yash Mantri (10429687), Tengyu He (4951795), Wonjun Yim (9706711), Ming Xu (151206), Zhuohong Wu (11184877), Pavla Fajtova (9279104), Matthew N. Creyer (10429690), Colman Moore (4715505), Lei Fu (429363), William F. Penny (3045048), Anthony J. O’Donoghue (7206356), Jesse V. Jokerst (801981) |
| سنة النشر: |
2022 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Medicine, Biotechnology, Immunology, Infectious Diseases, Virology, Biological Sciences not elsewhere classified, varying cysteine valence, tcep )- aunps, low nanomolar concentrations, interface gold colloids, identify individuals infected, exhaled breath matrices, cys )< sub, carboxyethyl ) phosphine, 17 ligated nanoparticles, one potential alternative, nucleic acid testing, tris ( 2, 2 viral replication, pro , x <, viral proteases, tris buffer, noninvasive alternative, care testing, 2 coronavirus, visual readout, subsequent sensing |
| الوصف: |
There is a need for surveillance of COVID-19 to identify individuals infected with SARS-CoV-2 coronavirus. Although specific, nucleic acid testing has limitations in terms of point-of-care testing. One potential alternative is the nonstructural protease (nsp5, also known as M pro /3CL pro ) implicated in SARS-CoV-2 viral replication but not incorporated into virions. Here, we report a divalent substrate with a novel design, (Cys) 2 –(AA) x –(Asp) 3 , to interface gold colloids in the specific presence of M pro leading to a rapid and colorimetric readout. Citrate- and tris(2-carboxyethyl)phosphine (TCEP)-AuNPs were identified as the best reporter out of the 17 ligated nanoparticles. Furthermore, we empirically determined the effects of varying cysteine valence and biological media on the sensor specificity and sensitivity. The divalent peptide was specific to M pro , that is, there was no response when tested with other proteins or enzymes. Furthermore, the M pro detection limits in Tris buffer and exhaled breath matrices are 12.2 and 18.9 nM, respectively, which are comparable to other reported methods (i.e., at low nanomolar concentrations) yet with a rapid and visual readout. These results from our work would provide informative rationales to design a practical and noninvasive alternative for COVID-19 diagnostic testingthe presence of viral proteases in biofluids is validated. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Peptidic_Sulfhydryl_for_Interfacing_Nanocrystals_and_Subsequent_Sensing_of_SARS-CoV_2_Protease/18657746 |
| DOI: |
10.1021/acs.chemmater.1c03871.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.chemmater.1c03871.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.94D1EE41 |
| قاعدة البيانات: |
BASE |