Academic Journal
Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis
| العنوان: | Requirements for F-BAR proteins TOCA-1 and TOCA-2 in actin dynamics and membrane trafficking during caenorhabditis elegans oocyte growth and embryonic epidermal morphogenesis |
|---|---|
| المؤلفون: | C. Giuliani, F. Troglio, Z. Bai, F. B. Patel, A. Zucconi1, A. Disanza, T. B. Stradal, G. Cassata, S. Confalonieri, J. D. Hardin, M. C. Soto, B. D. Grant, M.G. Malabarba, G. Scita |
| المساهمون: | C. Giuliani, F. Troglio, Z. Bai, F.B. Patel, A. Zucconi1, M.G. Malabarba, A. Disanza, T.B. Stradal, G. Cassata, S. Confalonieri, J.D. Hardin, M.C. Soto, B.D. Grant, G. Scita |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2009 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | Settore MED/04 - Patologia Generale |
| الوصف: | The TOCA family of F-BAR–containing proteins bind to and remodel lipid bilayers via their conserved F-BAR domains, and regulate actin dynamics via their N-Wasp binding SH3 domains. Thus, these proteins are predicted to play a pivotal role in coordinating membrane traffic with actin dynamics during cell migration and tissue morphogenesis. By combining genetic analysis in Caenorhabditis elegans with cellular biochemical experiments in mammalian cells, we showed that: i) loss of CeTOCA proteins reduced the efficiency of Clathrin-mediated endocytosis (CME) in oocytes. Genetic interference with CeTOCAs interacting proteins WSP-1 and WVE-1, and other components of the WVE-1 complex, produced a similar effect. Oocyte endocytosis defects correlated well with reduced egg production in these mutants. ii) CeTOCA proteins localize to cell–cell junctions and are required for proper embryonic morphogenesis, to position hypodermal cells and to organize junctional actin and the junction-associated protein AJM-1. iii) Double mutant analysis indicated that the toca genes act in the same pathway as the nematode homologue of N-WASP/WASP, wsp-1. Furthermore, mammalian TOCA-1 and C. elegans CeTOCAs physically associated with N-WASP and WSP-1 directly, or WAVE2 indirectly via ABI-1. Thus, we propose that TOCA proteins control tissues morphogenesis by coordinating Clathrin-dependent membrane trafficking with WAVE and NWASP– dependent actin-dynamics |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/19798448; info:eu-repo/semantics/altIdentifier/wos/WOS:000272032100013; volume:5; issue:10; firstpage:e1000675; journal:PLOS GENETICS; http://hdl.handle.net/2434/71812; http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000675 |
| DOI: | 10.1371/journal.pgen.1000675 |
| الاتاحة: | http://hdl.handle.net/2434/71812 https://doi.org/10.1371/journal.pgen.1000675 http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1000675 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9478FFEB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1371/journal.pgen.1000675 |
|---|