Academic Journal
Epigenetic changes in the early stage of silica-induced cell transformation
| العنوان: | Epigenetic changes in the early stage of silica-induced cell transformation |
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| المؤلفون: | Seidel, Carole, Kirsch, Anaïs, Fontana, Caroline, Visvikis, Athanase, Rémy, Aurélie, Gaté, Laurent, Darne, Christian, Guichard, Yves |
| المساهمون: | Département Toxicologie et biométrologie (TB), Institut national de recherche et de sécurité (Vandoeuvre lès Nancy) (INRS ( Vandoeuvre lès Nancy)), Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1743-5390. |
| بيانات النشر: | HAL CCSD Taylor & Francis |
| سنة النشر: | 2017 |
| المجموعة: | Université de Lorraine: HAL |
| مصطلحات موضوعية: | Promotion assay, Silica particles, C-myc expression, Epigenetic modifications, CYTOTOXICITE, ETAT AMORPHE, SILICE, RECHERCHE, CANCEROGENICITE, EXPERIMENTATION, GENOTOXICITE, PRODUIT CHIMIQUE, ETAT CRISTALLIN, [SDV.TOX]Life Sciences [q-bio]/Toxicology, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; The increasing use of nanomaterials in numerous domains has led to growing concern about their potential toxicological properties, and the potential risk to human health posed by silica nanoparticles remains under debate. Recent studies proposed that these particles could alter gene expression through the modulation of epigenetic marks, and the possible relationship between particle exposure and these mechanisms could represent a critical factor in carcinogenicity. In this study, using the Bhas 42 cell model, we compare the effects of exposure to two transforming particles, a pyrogenic amorphous silica nanoparticle NM-203 to those of the crystalline silica particle Min-U-Sil® 5. Short-term treatment by Min-U-Sil® 5 decreased global DNA methylation and increased the expression of the two de novo DNMTs, DNMT3a and DNMT3b. NM-203 treatment affected neither the expression of these enzymes nor DNA methylation. Moreover, modified global histone H4 acetylation status and HDAC protein levels were observed only in the Min-U-Sil® 5-treated cells. Finally, both types of particle treatment induced strong c-Myc expression in the early stage of cell transformation and this correlated with enrichment in RNA polymerase II as well as histone active marks on its promoter. Lastly, almost all parameters that were modulated in the early stage were restored in transformed cells suggesting their involvement mainly in the first steps of cell transformation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-01715151; https://hal.science/hal-01715151; https://hal.science/hal-01715151/document; https://hal.science/hal-01715151/file/nano_17435390.1382599.pdf |
| DOI: | 10.1080/17435390.2017.1382599 |
| الاتاحة: | https://hal.science/hal-01715151 https://hal.science/hal-01715151/document https://hal.science/hal-01715151/file/nano_17435390.1382599.pdf https://doi.org/10.1080/17435390.2017.1382599 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.93E7B92F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1080/17435390.2017.1382599 |
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