Academic Journal
Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
| العنوان: | Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus |
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| المؤلفون: | Visiedo, Francisco, Santos-Rosendo, Celeste, Mateos-Bernal, Rosa M., del Mar Gil-Sanchez, M., Bugatto, Fernando, Aguilar-Diosdado, Manuel, Segundo, Carmen, Lopez-Tinoco, Cristina |
| المساهمون: | Visiedo, Francisco Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, Santos-Rosendo, Celeste Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, Mateos-Bernal, Rosa M. Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, del Mar Gil-Sanchez, M. Univ Seville, Fac Biol, Dept Genet, Seville, Spain, Bugatto, Fernando Puerta Mar Univ Hosp, Dept Obstet & Gynecol, Cadiz, Spain, Lopez-Tinoco, Cristina Puerta Mar Univ Hosp, Dept Obstet & Gynecol, Cadiz, Spain, Aguilar-Diosdado, Manuel Puerta Mar Univ Hosp, Dept Endocrinol & Nutr, Cadiz, Spain, Segundo, Carmen Univ Cadiz, Fac Nursing, Salus Infirmorum, Cadiz, Spain, Andalusia Department of Health, PAIDI |
| بيانات النشر: | Hindawi ltd |
| سنة النشر: | 2017 |
| المجموعة: | Sistema Sanitario Público de Andalucía (SSPA): Repositorio |
| مصطلحات موضوعية: | Protein s-nitrosylation, Nitric-oxide, Oxidative stress, Apoptosis, Pathophysiology, Trophoblasts, Expression, Erk1/2, P38 |
| الوصف: | Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n = 8) and GDM (n = 8) pregnancies. S-Nitrosylation was measured using the biotin-switch assay, while the expression and protein activity were assessed by immunoblotting and colorimetric methods, respectively. Results indicated that catalase and peroxiredoxin nitrosylation levels were greater in GDM placentas, and that was accompanied by reduced catalase activity. S-Nitrosylation of ERK1/2 and AKT was increased in GDM placentas, and their activities were inhibited. Activities of caspase-3 and caspase-9 were increased, with the latter also showing diminished nitrosylation levels. These findings suggest that S-nitrosylation is a little-known, but critical, mechanism by which NO directlymodulates key placental proteins in women with GDM and, as a consequence, maternal and fetal anomalies during pregnancy can occur. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1942-0900 1942-0994 |
| Relation: | http://hdl.handle.net/10668/19062; http://downloads.hindawi.com/journals/omcl/2017/5629341.pdf; 398252200001 |
| DOI: | 10.1155/2017/5629341 |
| الاتاحة: | http://hdl.handle.net/10668/19062 https://doi.org/10.1155/2017/5629341 http://downloads.hindawi.com/journals/omcl/2017/5629341.pdf |
| Rights: | Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; open access |
| رقم الانضمام: | edsbas.9335712 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19420900 19420994 |
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| DOI: | 10.1155/2017/5629341 |