Academic Journal
Antagomir-mediated suppression of microRNA-134 reduces kainic acid-induced seizures in immature mice
| العنوان: | Antagomir-mediated suppression of microRNA-134 reduces kainic acid-induced seizures in immature mice |
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| المؤلفون: | Aoife Campbell, Gareth Morris, Janosch P. Heller, Elena Langa, Elizabeth Brindley, Jesper Worm, Mads Aaboe Jensen, Meghan T. Miller, David C. Henshall, Cristina R. Reschke |
| المصدر: | Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| بيانات النشر: | Nature Portfolio |
| سنة النشر: | 2021 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | MicroRNAs are short non-coding RNAs that negatively regulate protein levels and perform important roles in establishing and maintaining neuronal network function. Previous studies in adult rodents have detected upregulation of microRNA-134 after prolonged seizures (status epilepticus) and demonstrated that silencing microRNA-134 using antisense oligonucleotides, termed antagomirs, has potent and long-lasting seizure-suppressive effects. Here we investigated whether targeting microRNA-134 can reduce or delay acute seizures in the immature brain. Status epilepticus was induced in 21 day-old (P21) male mice by systemic injection of 5 mg/kg kainic acid. This triggered prolonged electrographic seizures and select bilateral neuronal death within the CA3 subfield of the hippocampus. Expression of microRNA-134 and functional loading to Argonaute-2 was not significantly changed in the hippocampus after seizures in the model. Nevertheless, when levels of microRNA-134 were reduced by prior intracerebroventricular injection of an antagomir, kainic acid-induced seizures were delayed and less severe and mice displayed reduced neuronal death in the hippocampus. These studies demonstrate targeting microRNA-134 may have therapeutic applications for the treatment of seizures in children. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doi.org/10.1038/s41598-020-79350-7; https://doaj.org/toc/2045-2322; https://doaj.org/article/0b672bed99a647c39d8f5224165e8622 |
| DOI: | 10.1038/s41598-020-79350-7 |
| الاتاحة: | https://doi.org/10.1038/s41598-020-79350-7 https://doaj.org/article/0b672bed99a647c39d8f5224165e8622 |
| رقم الانضمام: | edsbas.930A14C8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-020-79350-7 |