Academic Journal
second coding exon of Tat in T cells
| العنوان: | second coding exon of Tat in T cells |
|---|---|
| المؤلفون: | Ulrich Mahlknecht, Isabelle Dichamp, Audrey Varin, Carine Van Lint, Georges Herbein |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://www.jleukbio.org/content/83/3/718.full.pdf. |
| المجموعة: | CiteSeerX |
| الوصف: | HIV-1 two-exon transactivator protein (Tat) is a 101-aa protein. We investigated the pos-sible contribution of the extreme C terminus of HIV-1 Tat to maximize nuclear transcription fac-tor NF-B activation, long terminal repeat (LTR) transactivation, and viral replication in T cells. C-terminal deletion and substitution mutants made with the infectious clone HIV-89.6 were assayed for their ability to transactivate NF-B-secreted alkaline phosphatase and HIV-1 LTR-luciferase re-porter constructs for low concentrations of Tat. A mutant infectious clone of HIV-89.6 engineered by introducing a stop codon at aa 72 in the Tat open-reading frame (HIVtatexon2) replicated at a sig-nificantly lower rate than the wild-type HIV-89.6 in phytohemagglutinin-A/IL-2-stimulated primary pe-ripheral blood lymphocytes. Altogether, our re-sults suggest a critical role for the glutamic acids at positions 92, 94, and 96 or lysines at positions 88, 89, and 90, present in the second encoding Tat exon in activating NF-B, transactivating the HIV-1 LTR and enhancing HIV-1 replication in T cells. J. Leukoc. Biol. 83: 718–727; 2008. Key Words: LTR stimulation viral reservoir AIDS pathogenesis |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.583.4629; http://www.jleukbio.org/content/83/3/718.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.583.4629 http://www.jleukbio.org/content/83/3/718.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.92DAEB88 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |