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Image_1_The prognostic value of whole-genome DNA methylation in response to Leflunomide in patients with Rheumatoid Arthritis.pdf
| العنوان: | Image_1_The prognostic value of whole-genome DNA methylation in response to Leflunomide in patients with Rheumatoid Arthritis.pdf |
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| المؤلفون: | Yulan Chen, Qiao Wang, Haina Liu, Lei Jin, Xin Feng, Bingbing Dai, Meng Chen, Fangran Xin, Tingting Wei, Bingqing Bai, Zhijun Fan, Jiahui Li, Yuxin Yao, Ruobing Liao, Jintao Zhang, Xiangnan Jin, Lingyu Fu |
| سنة النشر: | 2023 |
| المجموعة: | Frontiers: Figshare |
| مصطلحات موضوعية: | Immunology, Applied Immunology (incl. Antibody Engineering, Xenotransplantation and T-cell Therapies), Autoimmunity, Cellular Immunology, Humoural Immunology and Immunochemistry, Immunogenetics (incl. Genetic Immunology), Innate Immunity, Transplantation Immunology, Tumour Immunology, Immunology not elsewhere classified, Genetic Immunology, Animal Immunology, Veterinary Immunology, rheumatoid arthritis, Leflunomide, methylation, machine learning, prognostic |
| الوصف: | Objective Although Leflunomide (LEF) is effective in treating rheumatoid arthritis (RA), there are still a considerable number of patients who respond poorly to LEF treatment. Till date, few LEF efficacy-predicting biomarkers have been identified. Herein, we explored and developed a DNA methylation-based predictive model for LEF-treated RA patient prognosis. Methods Two hundred forty-five RA patients were prospectively enrolled from four participating study centers. A whole-genome DNA methylation profiling was conducted to identify LEF-related response signatures via comparison of 40 samples using Illumina 850k methylation arrays. Furthermore, differentially methylated positions (DMPs) were validated in the 245 RA patients using a targeted bisulfite sequencing assay. Lastly, prognostic models were developed, which included clinical characteristics and DMPs scores, for the prediction of LEF treatment response using machine learning algorithms. Results We recognized a seven-DMP signature consisting of cg17330251, cg19814518, cg20124410, cg21109666, cg22572476, cg23403192, and cg24432675, which was effective in predicting RA patient’s LEF response status. In the five machine learning algorithms, the support vector machine (SVM) algorithm provided the best predictive model, with the largest discriminative ability, accuracy, and stability. Lastly, the AUC of the complex model(the 7-DMP scores with the lymphocyte and the diagnostic age) was higher than the simple model (the seven-DMP signature, AUC:0.74 vs 0.73 in the test set). Conclusion In conclusion, we constructed a prognostic model integrating a 7-DMP scores with the clinical patient profile to predict responses to LEF treatment. Our model will be able to effectively guide clinicians in determining whether a patient is LEF treatment sensitive or not. |
| نوع الوثيقة: | still image |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/figure/Image_1_The_prognostic_value_of_whole-genome_DNA_methylation_in_response_to_Leflunomide_in_patients_with_Rheumatoid_Arthritis_pdf/24099648 |
| DOI: | 10.3389/fimmu.2023.1173187.s001 |
| الاتاحة: | https://doi.org/10.3389/fimmu.2023.1173187.s001 https://figshare.com/articles/figure/Image_1_The_prognostic_value_of_whole-genome_DNA_methylation_in_response_to_Leflunomide_in_patients_with_Rheumatoid_Arthritis_pdf/24099648 |
| Rights: | CC BY 4.0 |
| رقم الانضمام: | edsbas.9258221B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2023.1173187.s001 |
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