Academic Journal
Large-scale transcriptomics to dissect 2 years of the life of a fungal phytopathogen interacting with its host plant
| العنوان: | Large-scale transcriptomics to dissect 2 years of the life of a fungal phytopathogen interacting with its host plant |
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| المؤلفون: | Gay, Elise, J, Soyer, Jessica, L, Lapalu, Nicolas, Linglin, Juliette, Fudal, Isabelle, da Silva, Corinne, Wincker, Patrick, Aury, Jean-Marc, Cruaud, Corinne, Levrel, Anne, Lemoine, Jocelyne, Delourme, Régine, Rouxel, Thierry, Balesdent, Marie-Hélène |
| المساهمون: | BIOlogie et GEstion des Risques en agriculture (BIOGER), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Bioinformatique pour la Génomique et la Biodiversité (LBGB), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage Evry (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Genoscope - Centre national de séquençage Evry (GENOSCOPE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Université de Rennes (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Promosol (METAPHOR project), France Genomique (Leptolife project), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) |
| المصدر: | ISSN: 1741-7007. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2021 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | Effectors, Expression waves, Chromatin regulation, Lifestyle transitions, Leptosphaeria maculans, Brassica napus, Fungal biology, Transcriptomics, Pathogenesis, [SDE]Environmental Sciences |
| الوصف: | International audience ; Background The fungus Leptosphaeria maculans has an exceptionally long and complex relationship with its host plant, Brassica napus, during which it switches between different lifestyles, including asymptomatic, biotrophic, necrotrophic, and saprotrophic stages. The fungus is also exemplary of "two-speed" genome organisms in the genome of which gene-rich and repeat-rich regions alternate. Except for a few stages of plant infection under controlled conditions, nothing is known about the genes mobilized by the fungus throughout its life cycle, which may last several years in the field. Results We performed RNA-seq on samples corresponding to all stages of the interaction of L. maculans with its host plant, either alive or dead (stem residues after harvest) in controlled conditions or in field experiments under natural inoculum pressure, over periods of time ranging from a few days to months or years. A total of 102 biological samples corresponding to 37 sets of conditions were analyzed. We show here that about 9% of the genes of this fungus are highly expressed during its interactions with its host plant. These genes are distributed into eight well-defined expression clusters, corresponding to specific infection lifestyles or to tissue-specific genes. All expression clusters are enriched in effector genes, and one cluster is specific to the saprophytic lifestyle on plant residues. One cluster, including genes known to be involved in the first phase of asymptomatic fungal growth in leaves, is re-used at each asymptomatic growth stage, regardless of the type of organ infected. The expression of the genes of this cluster is repeatedly turned on and off during infection. Whatever their expression profile, the genes of these clusters are enriched in heterochromatin regions associated with H3K9me3 or H3K27me3 repressive marks. These findings provide support for the hypothesis that part of the fungal genes involved in niche adaptation is located in heterochromatic regions of the genome, ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/33757516; hal-03190704; https://hal.inrae.fr/hal-03190704; https://hal.inrae.fr/hal-03190704/document; https://hal.inrae.fr/hal-03190704/file/Gay-2021-Large-scale%20transcriptomics%20to%20dissect.pdf; PUBMED: 33757516; WOS: 000632066600001 |
| DOI: | 10.1186/s12915-021-00989-3 |
| الاتاحة: | https://hal.inrae.fr/hal-03190704 https://hal.inrae.fr/hal-03190704/document https://hal.inrae.fr/hal-03190704/file/Gay-2021-Large-scale%20transcriptomics%20to%20dissect.pdf https://doi.org/10.1186/s12915-021-00989-3 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.923EBA57 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12915-021-00989-3 |
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