Academic Journal
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of Moraxella bovoculi and Moraxella bovis isolates from cattle
| العنوان: | Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of Moraxella bovoculi and Moraxella bovis isolates from cattle |
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| المؤلفون: | Robbins, Kara, Dickey, Aaron M., Clawson, Michael L., Loy, John D. |
| المساهمون: | Agricultural Research Division, Institute of Agriculture and Natural Resources, National Institute of Food and Agriculture |
| المصدر: | Journal of Veterinary Diagnostic Investigation ; volume 30, issue 5, page 739-742 ; ISSN 1040-6387 1943-4936 |
| بيانات النشر: | SAGE Publications |
| سنة النشر: | 2018 |
| الوصف: | Infectious bovine keratoconjunctivitis (IBK) is an economically significant disease caused by Moraxella bovis. Moraxella bovoculi, although not reported to cause IBK, has been isolated from the eyes of cattle diagnosed with IBK. Identification of M. bovis and M. bovoculi can be performed using biochemical or DNA-based approaches, both of which may be time consuming and inconsistent between laboratories. We conducted a comparative evaluation of M. bovoculi and M. bovis identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) with a database provided by Bruker Daltonics (termed the BDAL database), the BDAL database supplemented with spectra generated in our study (termed the UNLVDC database), and with PCR–restriction-fragment length polymorphism (PCR-RFLP) typing. M. bovoculi ( n = 250) and M. bovis ( n = 18) isolates from cattle with or without IBK were used. MALDI-TOF MS using the UNLVDC database correctly identified 250 of 250 (100%) of M. bovoculi and 17 of 18 (94%) of M. bovis isolates. With the BDAL database, MALDI-TOF MS correctly identified 249 of 250 (99%) of M. bovoculi and 7 of 18 (39%) of M. bovis isolates. In comparison, the PCR-RFLP test correctly identified 210 of 250 (84%) of M. bovoculi and 12 of 18 (66%) of M. bovis isolates. Thus, MALDI-TOF MS with the UNLVDC database was the most effective identification methodology for M. bovis and M. bovoculi isolates from cattle. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1177/1040638718789725 |
| الاتاحة: | https://doi.org/10.1177/1040638718789725 https://journals.sagepub.com/doi/pdf/10.1177/1040638718789725 https://journals.sagepub.com/doi/full-xml/10.1177/1040638718789725 |
| Rights: | http://www.sagepub.com/licence-information-for-chorus |
| رقم الانضمام: | edsbas.92168A86 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1177/1040638718789725 |
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