Academic Journal
Preparation and Characterization of Resveratrol Loaded Pectin/Alginate Blend Gastro-Resistant Microparticles
| العنوان: | Preparation and Characterization of Resveratrol Loaded Pectin/Alginate Blend Gastro-Resistant Microparticles |
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| المؤلفون: | Gartziandia López de Goikoetxea, Oihane, Lasa Elguezua, Arrate, Pedraz Muñoz, José Luis, Miranda Gómez, Jonatan, Portillo Baquedano, María Puy, Igartua Olaechea, Manuela, Hernández Martín, Rosa María |
| بيانات النشر: | MDPI |
| سنة النشر: | 2018 |
| المجموعة: | ADDI: Repositorio Institucional de la Universidad del País Vasco / Euskal Herriko Unibertsitatea (UPV/EHU - Basque Country University) |
| مصطلحات موضوعية: | resveratrol, dietary supplement, gastro-resistant, microparticles, obesity, HPLC, fat mobilization, supplementation, bioavailability, encapsulation, absorption, delivery, humans, liver, rats |
| الوصف: | Background: The use of resveratrol as a dietary supplement is limited because it is easily oxidized and, after oral ingestion, it is metabolized into enterocytes and hepatocytes. Thus, new formulations are needed in order to improve its oral bioavailability. Objective: The objective of this study was to develop and characterize a gastro-resistant formulation of resveratrol for oral administration as a dietary supplement. Method: Resveratrol was encapsulated in Eudragit-coated pectin-alginate microparticles. Results: The microparticle size was about 1450 mu m, with an encapsulation efficiency of 41.72% +/- 1.92%. The dissolution assay conducted, as specified in the European Pharmacopoeia for delayed-release dosage forms, revealed that our microparticles were gastro-resistant, because the resveratrol percentage released from microparticles in acid medium was less than 10%. In addition, the high-performance liquid chromatographic (HPLC) method developed for resveratrol content quantification in the microparticles was validated according to International Council for Harmonisation (ICH) Q2 (R1) guidelines. Finally, the biological activity of resveratrol was investigated in 3T3-L1 mature adipocytes, concluding that the encapsulation process does not affect the activity of resveratrol. Conclusion: In summary, the gastro-resistant microparticles developed could represent a suitable method of including resveratrol in dietary supplements and in functional foods used in obesity therapy. ; This research was partially funded by the Basque Government (Consolidated Groups, IT-407-07; IT-572-13; SAIOTEK SA-2011-00118) and the Spanish Government (INNPACTO, IPT-2012-0602-300000, 2012). The authors gratefully acknowledge the support provided by the University of the Basque Country UPV/EHU (UFI11/32). |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| Relation: | https://www.mdpi.com/1420-3049/23/8/1886; Molecules 23(8) : (2018) // Article ID 1886; http://hdl.handle.net/10810/30250 |
| DOI: | 10.3390/molecules23081886 |
| الاتاحة: | http://hdl.handle.net/10810/30250 https://doi.org/10.3390/molecules23081886 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/3.0/es/ ; This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). ; Atribución 3.0 España |
| رقم الانضمام: | edsbas.919BCBD |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14203049 |
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| DOI: | 10.3390/molecules23081886 |