Academic Journal
Acute ischemic stroke thrombi have an outer shell that impairs fibrinolysis
| العنوان: | Acute ischemic stroke thrombi have an outer shell that impairs fibrinolysis |
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| المؤلفون: | Meglio, Lucas, Di, Desilles, Jean-Philippe, Ollivier, Véronique, Nomenjanahary Msci, Mialitiana Solo, Di Meglio, Sara, Deschildre, Catherine, Loyau, Stéphane, Olivot, Jean-Marc, Blanc, Raphaël, Piotin, Michel, Bouton, Marie-Christine, Michel, Jean-Baptiste, Jandrot-Perrus, Martine, Ho-Tin-Noe, Benoît, Mazighi, Mikael |
| المساهمون: | Laboratoire de Recherche Vasculaire Translationnelle (LVTS (UMR_S_1148 / U1148)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, CHU Rothschild AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), This work was supported by INSERM, La Fondation pour la Recherche sur les AVC (grant # FR-AVC-003), La Fondation pour la Recherche Médicale (grant #DPC20171138959), Fondation de l’Avenir and by the French Agence Nationale de la Recherche ANR-16-RHUS0004 (RHU TRT_cSVD), and BPI (CMI2 project TherAVC2.0). Lucas Di Meglio is the recipient of a PhD grant from La Fondation de L’Avenir., ANR-16-RHUS-0004,TRT-cSVD,TRT-cSVD(2016) |
| المصدر: | ISSN: 0028-3878. |
| بيانات النشر: | HAL CCSD American Academy of Neurology |
| سنة النشر: | 2019 |
| المجموعة: | Université Paris 13: HAL |
| مصطلحات موضوعية: | shell Publication history: none Submission Type: Article Title character count: 75 Number of Tables : 1 + 1 supplemental, Search Terms: Stroke, thrombus histology, thrombus structure, thrombolysis, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology |
| الوصف: | Erratum inAcute ischemic stroke thrombi have an outer shell that impairs fibrinolysis. [Neurology. 2019] ; International audience ; OBJECTIVES:Thrombi responsible for large vessel occlusion (LVO) in the setting of acute ischemic stroke (AIS) are characterized by a low recanalization rate after IV thrombolysis. To test whether AIS thrombi have inherent common features that limit their susceptibility to thrombolysis, we analyzed the composition and ultrastructural organization of AIS thrombi causing LVO.METHODS:A total of 199 endovascular thrombectomy-retrieved thrombi were analyzed by immunohistology and scanning electron microscopy (SEM) and subjected to ex vivo thrombolysis assay. The relationship between thrombus organization and thrombolysis resistance was further investigated in vitro using thrombus produced by recalcification of citrated whole blood.RESULTS:SEM and immunohistology analyses revealed that, although AIS thrombus composition and organization was highly heterogeneous, AIS thrombi shared a common remarkable structural feature in the form of an outer shell made of densely compacted thrombus components including fibrin, von Willebrand factor, and aggregated platelets. In vitro thrombosis experiments using human blood indicated that platelets were essential to the formation of the thrombus outer shell. Finally, in both AIS and in vitro thrombi, the thrombus outer shell showed a decreased susceptibility to tissue plasminogen activator-mediated thrombolysis as compared to the thrombus inner core.INTERPRETATION:Irrespective of their etiology and despite their heterogeneity, intracranial thrombi causing LVO have a core shell structure that influences their susceptibility to thrombolysis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/31541014; inserm-02557253; https://inserm.hal.science/inserm-02557253; https://inserm.hal.science/inserm-02557253/document; https://inserm.hal.science/inserm-02557253/file/Neurology%20Di%20Meglio%20revised%20HAL.pdf; PUBMED: 31541014; PUBMEDCENTRAL: PMC6946479 |
| DOI: | 10.1212/WNL.0000000000008395 |
| الاتاحة: | https://inserm.hal.science/inserm-02557253 https://inserm.hal.science/inserm-02557253/document https://inserm.hal.science/inserm-02557253/file/Neurology%20Di%20Meglio%20revised%20HAL.pdf https://doi.org/10.1212/WNL.0000000000008395 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.90E20D62 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1212/WNL.0000000000008395 |
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