Academic Journal
IL‐2mAb reduces demyelination after focal cerebral ischemia by suppressing CD8 + T cells
| العنوان: | IL‐2mAb reduces demyelination after focal cerebral ischemia by suppressing CD8 + T cells |
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| المؤلفون: | Zhou, Yu‐Xi, Wang, Xin, Tang, Dan, Li, Yan, Jiao, Ying‐Fu, Gan, Yu, Hu, Xiao‐Ming, Yang, Li‐Qun, Yu, Wei‐Feng, Stetler, Ruth Anne, Li, Pei‐Ying, Wen, Da‐Xiang |
| المساهمون: | Science and Technology Commission of Shanghai Municipality, Shanghai Municipal Education Commission, National Natural Science Foundation of China |
| المصدر: | CNS Neuroscience & Therapeutics ; volume 25, issue 4, page 532-543 ; ISSN 1755-5930 1755-5949 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary Aims Demyelination, one of the major pathological changes of white matter injury, is closely related to T‐cell–mediated immune responses. Thus, we investigate the role of an IL‐2 monoclonal antibody (IL‐2mAb, JES6‐1) in combatting demyelination during the late phase of stroke. Methods IL‐2mAb or IgG isotype antibody (0.25 mg/kg) was injected intraperitoneally 2 and 48 hours after middle cerebral artery occlusion (MCAO) surgery. Infarct volume, peripheral immune cell infiltration, microglia activation, and myelin loss were measured by 2,3,5‐triphenyte trazoliumchloride staining, immunofluorescence staining, flow cytometry, and Western blot. Intraperitoneal CD8 neutralizing antibody (15 mg/kg) was injected 1 day before MCAO surgery to determine the role of CD8 + T cells on demyelinating lesions. Results IL‐2mAb treatment reduced brain infarct volume, attenuated demyelination, and improved long‐term sensorimotor functions up to 28 days after dMCAO. Brain infiltration of CD8 + T cells and peripheral activation of CD8 + T cells were both attenuated in IL‐2 mAb‐treated mice. The protection of IL‐2mAb on demyelination was abolished in mice depleted of CD8 + T cell 1 week after stroke. Conclusions IL‐2mAb preserved white matter integrity and improved long‐term sensorimotor functions following cerebral ischemic injury. The activation and brain infiltration of CD8 + T cells are detrimental for demyelination after stroke and may be the major target of IL‐2mAb posttreatment in the protection of white matter integrity after stroke. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/cns.13084 |
| الاتاحة: | http://dx.doi.org/10.1111/cns.13084 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fcns.13084 https://onlinelibrary.wiley.com/doi/pdf/10.1111/cns.13084 https://onlinelibrary.wiley.com/doi/full-xml/10.1111/cns.13084 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.90A094D1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/cns.13084 |
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