Academic Journal
Mesenchymal Stem Cells Induce Suppressive Macrophages through Phagocytosis in a Mouse Model of Asthma
| العنوان: | Mesenchymal Stem Cells Induce Suppressive Macrophages through Phagocytosis in a Mouse Model of Asthma |
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| المؤلفون: | Braza, Faouzi, Dirou, Stéphanie, Forest, Virginie, Sauzeau, Vincent, Hassoun, Dorian, Chesné, Julie, Cheminant‐muller, Marie‐aude, Sagan, Christine, Magnan, Antoine, Lemarchand, Patricia |
| المساهمون: | ITX - unité de recherche de l'institut du thorax (ITX), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), This work was supported by a grant from Institut de Recherche en SantéRespiratoire des Pays de la Loire (France). |
| المصدر: | ISSN: 1066-5099 ; STEM CELLS ; https://inserm.hal.science/inserm-01277384 ; STEM CELLS, 2016, ⟨10.1002/stem.2344⟩. |
| بيانات النشر: | CCSD AlphaMed Press |
| سنة النشر: | 2016 |
| المجموعة: | Université de Nantes: HAL-UNIV-NANTES |
| مصطلحات موضوعية: | house dust mite asthma, mesenchymal stem cells, M2 macrophage, airway hyperresponsiveness, phagocytosis, airway smooth muscle contraction, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | International audience ; ac‐ cepted for publication February 01, 2016; available online without sub‐ scription through the open access option. ©AlphaMed Press 1066‐5099/2016/$30.00/0 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typeset‐ ting, pagination and proofreading process which may lead to differ‐ ences between this version and the Version of Record. Please cite this article as 1,2,3,4 Key words. house dust mite asthma mesenchymal stem cells M2 macro‐ phage airway hyper‐responsiveness phagocytosis airway smooth muscle contraction. ABSTRACT Mesenchymal stem cell (MSC) immunosuppressive functions make them attractive candidates for anti‐inflammatory therapy in allergic asthma. However the mechanisms by which they ensure therapeutic effects remain to be elucidated. In an acute mouse model of house dust mite (Der f)‐ induced asthma, one i.v. MSC injection was sufficient to normalize and stabilize lung function in Der f‐sensitized mice as compared to control mice. MSC injection decreased in vivo airway responsiveness and de‐ creased ex vivo carbachol‐induced bronchial contraction, maintaining bronchial expression of the inhibitory type 2 muscarinic receptor. To eval‐ uate in vivo MSC survival, MSCs were labelled with PKH26 fluorescent marker prior to i.v. injection, and 1 to 10 days later total lungs were digest‐ ed to obtain single‐cell suspensions. 91.5 ± 2.3% and 86.6 ± 6.3% of the recovered PKH26 + lung cells expressed specific macrophage markers in control and Der f mice respectively, suggesting that macrophages had phagocyted in vivo the injected MSCs. Interestingly, only PKH26 + macro‐ phages expressed M2 phenotype, while the innate PKH26 ‐ macrophages expressed M1 phenotype. Finally, the remaining 0.5% PKH26 + MSCs ex‐ pressed 10 to 100 fold more COX‐2 than before injection, suggesting in vivo MSC phenotype modification. Together, the results of this study indicate that MSCs attenuate asthma by being phagocyted by lung ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1002/stem.2344 |
| الاتاحة: | https://inserm.hal.science/inserm-01277384 https://inserm.hal.science/inserm-01277384v1/document https://inserm.hal.science/inserm-01277384v1/file/fichier%20pour%20HAL.pdf https://inserm.hal.science/inserm-01277384v1/file/Braza_et_al-2016-STEM_CELLS.pdf https://doi.org/10.1002/stem.2344 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.905318C3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1002/stem.2344 |
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