Academic Journal
Method for Large-scale Production of hIPSC Spheroids
| العنوان: | Method for Large-scale Production of hIPSC Spheroids |
|---|---|
| المؤلفون: | Lemarié, Lucas, Courtial, Edwin-Joffrey, Sohier, Jérôme |
| المساهمون: | SEGULA Technologies Trappes, Segula Technologies France, Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2331-8325 ; Bio-protocol ; https://hal.science/hal-04536259 ; Bio-protocol , 2024, 14 (1342), ⟨10.21769/BioProtoc.4965⟩. |
| بيانات النشر: | HAL CCSD Bio-protocol LCC |
| سنة النشر: | 2024 |
| المجموعة: | Université de Lyon: HAL |
| مصطلحات موضوعية: | Spheroids Induced pluripotent stem cell Large-scale Uniform Autoclavable Tissue engineering, Spheroids, Induced pluripotent stem cell, Large-scale, Uniform, Autoclavable, Tissue engineering, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] |
| الوصف: | International audience ; Stem cell spheroids are rapidly becoming essential tools for a diverse array of applications ranging from tissue engineering to 3D cell models and fundamental biology. Given the increasing prominence of biotechnology, there is a pressing need to develop more accessible, efficient, and reproducible methods for producing these models. Various techniques such as hanging drop, rotating wall vessel, magnetic levitation, or microfluidics have been employed to generate spheroids. However, none of these methods facilitate the easy and efficient production of a large number of spheroids using a standard 6-well plate. Here, we present a novel method based on pellet culture (utilizing U-shapedmicrostructures) using a silicon mold produced through 3D printing, along with a detailed and illustrated manufacturing protocol. This technique enables the rapid production of reproducible and controlled spheroids (for 1 × 106 cells, spheroids = 130 ± 10 μm) from human induced pluripotent stem cells (hIPSCs) within a short time frame (24 h). Importantly, the method allows the production of large quantities (2 × 104 spheroids for 1 × 106 cells) in an accessible and cost-effective manner, thanks to the use of a reusable mold. The protocols outlined herein are easily implementable, and all the necessary files for the method replication are freely available. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | hal-04536259; https://hal.science/hal-04536259; https://hal.science/hal-04536259/document; https://hal.science/hal-04536259/file/Bio-protocol4965.pdf |
| DOI: | 10.21769/BioProtoc.4965 |
| الاتاحة: | https://hal.science/hal-04536259 https://hal.science/hal-04536259/document https://hal.science/hal-04536259/file/Bio-protocol4965.pdf https://doi.org/10.21769/BioProtoc.4965 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.9046FE91 |
| قاعدة البيانات: | BASE |
| DOI: | 10.21769/BioProtoc.4965 |
|---|