Academic Journal
Analysis of repair and PCNA complex formation induced by ionizing radiation in human fibroblast cell lines
| العنوان: | Analysis of repair and PCNA complex formation induced by ionizing radiation in human fibroblast cell lines |
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| المؤلفون: | Karmakar, Parimal, Balajee, A.S., Natarajan, A.T. |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2001 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | ORIGINAL ARTICLES |
| الوصف: | Proliferating cell nuclear antigen (PCNA), an auxiliary factor for DNA polymerase δ and ε, is involved in both DNA replication and repair. Previous studies in vitro have demonstrated the requirement of PCNA in the resynthesis step of nucleotide excision repair (NER) and base excision repair (BER). Using a native chromatin template isolated under near physiological conditions, we have analysed the involvement of PCNA in the BER pathway in different NER defective human cell lines. The repair sites and PCNA were visualized by indirect immunolabelling followed by fluorescence microscopy. The results indicate that exposure to X-rays triggers the induction of PCNA in all the three human fibroblast cell lines studied, namely normal, xeroderma pigmentosum group A (XP-A) and Cockayne syndrome group B (CS-B). In all the cell lines, induction of PCNA and repair patches occurred in a dose- and time-dependent fashion. Induction of repair patches in NER-deficient XP-A cells suggests that the X-ray-induced lesions are largely repaired via the BER pathway involving PCNA as one of the key components of this pathway. X-ray-induced repair synthesis was greatly inhibited by treatment of cells with DNA polymerase inhibitors aphidicolin and cytosine arabinoside. Interestingly, inhibition of repair resynthesis did not affect the intensity of PCNA staining in X-irradiated cells indicating that the PCNA may be required for the BER pathway at a step preceding the resynthesis step. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://mutage.oxfordjournals.org/cgi/content/short/16/3/225; http://dx.doi.org/10.1093/mutage/16.3.225 |
| DOI: | 10.1093/mutage/16.3.225 |
| الاتاحة: | http://mutage.oxfordjournals.org/cgi/content/short/16/3/225 https://doi.org/10.1093/mutage/16.3.225 |
| Rights: | Copyright (C) 2001, Oxford University Press |
| رقم الانضمام: | edsbas.903D4B1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/mutage/16.3.225 |
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