Academic Journal
Increased Presence of Circulating Cell-Free, Fragmented, Host DNA in Pigs Infected with Virulent African Swine Fever Virus
| العنوان: | Increased Presence of Circulating Cell-Free, Fragmented, Host DNA in Pigs Infected with Virulent African Swine Fever Virus |
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| المؤلفون: | Olesen, Ann Sofie, Lohse, Louise, Johnston, Camille Melissa, Rasmussen, Thomas Bruun, Bøtner, Anette, Belsham, Graham J. |
| المصدر: | Olesen , A S , Lohse , L , Johnston , C M , Rasmussen , T B , Bøtner , A & Belsham , G J 2023 , ' Increased Presence of Circulating Cell-Free, Fragmented, Host DNA in Pigs Infected with Virulent African Swine Fever Virus ' , Viruses , vol. 15 , no. 10 , 2133 . https://doi.org/10.3390/v15102133 |
| سنة النشر: | 2023 |
| المجموعة: | University of Copenhagen: Research / Forskning ved Københavns Universitet |
| مصطلحات موضوعية: | African swine fever virus, apoptosis, biomarker, cell-free DNA, mitochondrial DNA |
| الوصف: | African swine fever virus (ASFV) causes severe hemorrhagic disease in domestic pigs and wild boar, often with high case fatality rates. The virus replicates in the circulating cells of the monocyte–macrophage lineage and within lymphoid tissues. The infection leads to high fever and a variety of clinical signs. In this study, it was observed that ASFV infection in pigs resulted in a >1000-fold increase in the level of circulating cell-free DNA (cfDNA), derived from the nuclei of host cells in the serum. This change occurred in parallel with the increase in circulating ASFV DNA. In addition, elevated levels (about 30-fold higher) of host mitochondrial DNA (mtDNA) were detected in the serum from ASFV-infected pigs. For comparison, the release of the cellular enzyme, lactate dehydrogenase (LDH), a commonly used marker of cellular damage, was also found to be elevated during ASFV infection, but later and less consistently. The sera from pigs infected with classical swine fever virus (CSFV), which causes a clinically similar disease to ASFV, were also tested but, surprisingly, this infection did not result in the release of cfDNA, mtDNA, or LDH. It was concluded that the level of cfDNA in the serum is a sensitive host marker of virulent ASFV infection. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| DOI: | 10.3390/v15102133 |
| الاتاحة: | https://researchprofiles.ku.dk/da/publications/increased-presence-of-circulating-cellfree-fragmented-host-dna-in-pigs-infected-with-virulent-african-swine-fever-virus(a116f7b3-232c-4c68-98d9-aad853556958).html https://doi.org/10.3390/v15102133 https://curis.ku.dk/ws/files/375056961/Increased_Presence_of_Circulating_Cell_Free_Fragmented_Host_DNA_in_Pigs_Infected_with_Virulent_African_Swine_Fever_Virus.pdf |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.900D40EC |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/v15102133 |
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