Academic Journal
Study protocol of the B-CAST study: a multicenter, prospective cohort study investigating the tumor biomarkers in adjuvant chemotherapy for stage III colon cancer
| العنوان: | Study protocol of the B-CAST study: a multicenter, prospective cohort study investigating the tumor biomarkers in adjuvant chemotherapy for stage III colon cancer |
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| المؤلفون: | Ishiguro, Megumi, Kotake, Kenjiro, Nishimura, Genichi, Tomita, Naohiro, Ichikawa, Wataru, Takahashi, Keiichi, Watanabe, Toshiaki, Furuhata, Tomohisa, Kondo, Ken, Mori, Masaki, Kakeji, Yoshihiro, Kanazawa, Akiyoshi, Kobayashi, Michiya, Okajima, Masazumi, Hyodo, Ichinosuke, Miyakoda, Keiko, Sugihara, Kenichi |
| المصدر: | BMC Cancer ; volume 13, issue 1 ; ISSN 1471-2407 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2013 |
| الوصف: | Background Adjuvant chemotherapy for stage III colon cancer is internationally accepted as standard treatment with established efficacy. Several oral fluorouracil (5-FU) derivatives with different properties are available in Japan, but which drug is the most appropriate for each patient has not been established. Although efficacy prediction of 5-FU derivatives using expression of 5-FU activation/metabolism enzymes in tumors has been studied, it has not been clinically applied. Methods/design The B-CAST study is a multicenter, prospective cohort study aimed to identify the patients who benefit from adjuvant chemotherapy with each 5-FU regimen, through evaluating the relationship between tumor biomarker expression and treatment outcome. The frozen tumor specimens of patients with stage III colon cancer who receives postoperative adjuvant chemotherapy are examined. Protein expression of thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD), epidermal growth factor receptor (EGFR), and vascular endothelial growth factor (VEGF) are evaluated using enzyme-linked immunosorbent assay (ELISA). mRNA expression of TP, DPD, thymidylate synthase (TS) and orotate phosphoribosyl transferase (OPRT) are evaluated using reverse transcription polymerase chain reaction (RT-PCR). The patients’ clinical data reviewed are as follow: demographic and pathological characteristics, regimen, drug doses and treatment duration of adjuvant therapy, types and severity of adverse events, disease free survival, relapse free survival and overall survival. Then, relationships among the protein/mRNA expression, clinicopathological characteristics and the treatment outcomes are analyzed for each 5-FU derivative. Discussion A total of 2,128 patients from the 217 institutions were enrolled between April 2009 and March 2012. The B-CAST study demonstrated that large-scale, multicenter translational research using frozen samples was feasible when the sample shipment and Web-based data collection were well organized. The results of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1186/1471-2407-13-149 |
| DOI: | 10.1186/1471-2407-13-149.pdf |
| DOI: | 10.1186/1471-2407-13-149/fulltext.html |
| الاتاحة: | http://dx.doi.org/10.1186/1471-2407-13-149 http://link.springer.com/content/pdf/10.1186/1471-2407-13-149.pdf http://link.springer.com/article/10.1186/1471-2407-13-149/fulltext.html http://link.springer.com/content/pdf/10.1186/1471-2407-13-149 https://link.springer.com/content/pdf/10.1186/1471-2407-13-149.pdf |
| Rights: | http://www.springer.com/tdm |
| رقم الانضمام: | edsbas.8FFB3331 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/1471-2407-13-149 |
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