Academic Journal
Presence and interaction of free-living amoebae and amoeba-resisting bacteria in water from drinking water treatment plants
| العنوان: | Presence and interaction of free-living amoebae and amoeba-resisting bacteria in water from drinking water treatment plants |
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| المؤلفون: | Gomes, Thiago Santos, Vaccaro Muñoz, Lucianna Rosalía, Magnet, Angela., Izquierdo Arias, Fernando, Ollero Baceiredo, Dolores, Martínez-Fernández, Carmen, Mayo Martinez, Laura, Moran Garrido, María., Pozuelo de Felipe, María José, Fenoy Rodríguez, Soledad., Hurtado Marcos, Carolina, Águila, Carmen del. |
| المساهمون: | Universidad San Pablo-CEU. Facultad de Farmacia. Departamento de Ciencias Farmacéuticas y de la Salud., Grupo: Parasitología e Inmunología molecular con aplicación biotecnológica, diagnóstica y terapéutica (PARINM), Universidad San Pablo-CEU. Facultad de Farmacia. |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Acanthamoeba, Amoeba-resisting bacteria, Drinking water, Free-living amoeba, Legionella, Spain |
| الوصف: | Acceso al texto completo en la siguiente URL: https://www.sciencedirect.com/science/article/pii/S0048969720305908 ; Este artículo está en acceso abierto siguiendo la política de la revista ; Free-living amoebae (FLA) are ubiquitous and many isolates have been shown to be infected with amoeba-resisting bacteria, as the example of Acanthamoeba and Legionella interaction. Due to the high environmental prevalence of Acanthamoeba. in the Castilian Plateau (Spain), the aims of this work were to investigate the occurrence of Acanthamoeba and other FLA in water from several sampling points from four Drinking Water Treatment Plants (DWTP) and to investigate the presence of Legionella spp. and other amoeba-resisting bacteria in biofilms in raw and finished water, taking into account that no legislation exists for this protozoa control. Acanthamoeba was detected at different sampling points, and sand filters seemed to contribute to amoebic enrichment. After ozonation, a temporary decrease in viable amoebae was observed. The genotypes detected were T3, T4, and T5, revealing the first report of genotype T5 in waters from this region. Moreover, Balamuthia mandrillaris, Vermamoeba vermiformis and Paravahlkampfia sp. were detected. Regarding Legionella, PCR detection in raw and finished water was higher than by agar culture, but even higher after Acanthamoeba co-culture. Also, Legionella's presence was higher in raw water than in finished water. The decrease of free Legionella observed from raw (27.5%, by PCR) to finished water (3.4% by PCR) contrasted with the increase of Legionella-infected FLA from raw (30.7%) to finished water (52%). At biofilm, free Legionella was not detected, and the percentage of infected FLA was low (3.8%). Legionella species identified in these samples were L. drozanskii, L. donaldsonii and L. feeleii. Additionally, Acanthamoeba co-culture led to the isolation of Pseudomonas aeruginosa, P. stutzeri, P. fluorecens, Achromobacter xylosoxidans and Stenotrophomonas maltophilia. The highly disseminated ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0048-9697 |
| Relation: | The Science of the total environment; Gomes TS, Vaccaro L, Magnet A, Izquierdo F, Ollero D, Martínez-Fernández C, Mayo L, Moran M, Pozuelo MJ, Fenoy S, Hurtado C, Del Águila C. Presence and interaction of free-living amoebae and amoeba-resisting bacteria in water from drinking water treatment plants. Sci Total Environ. 2020 Jun 1;719:137080. doi:10.1016/j.scitotenv.2020.137080.; http://hdl.handle.net/10637/15035 |
| DOI: | 10.1016/j.scitotenv.2020.137080 |
| الاتاحة: | http://hdl.handle.net/10637/15035 https://doi.org/10.1016/j.scitotenv.2020.137080 |
| رقم الانضمام: | edsbas.8FF44D60 |
| قاعدة البيانات: | BASE |
| تدمد: | 00489697 |
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| DOI: | 10.1016/j.scitotenv.2020.137080 |