Academic Journal
Selection of similar single domain antibodies from two immune VHH libraries obtained from two alpacas by using different selection methods.
| العنوان: | Selection of similar single domain antibodies from two immune VHH libraries obtained from two alpacas by using different selection methods. |
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| المؤلفون: | Li, Tengfei, Vandesquille, Matthias, Bay, Sylvie, Dhenain, Marc, Delatour, Benoît, Lafaye, Pierre |
| المساهمون: | Université Paris Diderot - Paris 7 (UPD7), Ingénierie des Anticorps (plate-forme) - Antibody Engineering (Platform), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Chimie des Biomolécules - Chemistry of Biomolecules, Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Maladies Neurodégénératives - UMR 9199 (LMN), Molecular Imaging Research Center Fontenay-aux-Roses (MIRCEN), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 0165-2478 ; Immunology Letters ; https://pasteur.hal.science/pasteur-01580563 ; Immunology Letters, 2017, 188, pp.89-95. ⟨10.1016/j.imlet.2017.07.001⟩. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Anti GFAP VHH, Phage display, Ribosome display, VH, VHH, [SDV.IMM]Life Sciences [q-bio]/Immunology |
| الوصف: | International audience ; The two most used methods to select camelid single-domain antibody-fragments (VHHs) are: displaying their repertoires on the surface of filamentous bacteriophages (phage display) or linking them to ribosomes (ribosome display). In this study, we compared specific VHHs isolated from two different immune libraries coming from two different alpacas by using these two selection methods. Three anti-GFAP (glial fibrillary acidic protein) VHHs were derived from an immune library obtained by ribosome display after immunization of one alpaca with purified GFAP, a protein expressed by astroglial cells. In parallel, three other anti-GFAP VHHs were derived from an immune library by phage display after immunization of another alpaca with a human brain tissue extract containing GFAP. All the VHHs were closely related and one VHH was found to be strictly identical in both studies. This highlights the selection pressure exerted by the camelid immune system to shape the paratope of an antibody against a defined antigen. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/28690185; pasteur-01580563; https://pasteur.hal.science/pasteur-01580563; https://pasteur.hal.science/pasteur-01580563/document; https://pasteur.hal.science/pasteur-01580563/file/1-s2.0-S0165247817301165-main%281%29.pdf; PUBMED: 28690185 |
| DOI: | 10.1016/j.imlet.2017.07.001 |
| الاتاحة: | https://pasteur.hal.science/pasteur-01580563 https://pasteur.hal.science/pasteur-01580563/document https://pasteur.hal.science/pasteur-01580563/file/1-s2.0-S0165247817301165-main%281%29.pdf https://doi.org/10.1016/j.imlet.2017.07.001 |
| Rights: | http://creativecommons.org/licenses/by-nc-sa/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.8F5C958F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.imlet.2017.07.001 |
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