التفاصيل البيبلوغرافية
| العنوان: |
Towards electrochemical sensor based on molecularly imprinted polypyrrole for the detection of bacteria-Listeria monocytogenes / |
| المؤلفون: |
Liustrovaitė, Viktorija, Pogorielov, Maksym, Bogužaitė, Raimonda, Ratautaitė, Vilma, Ramanavičienė, Almira, Pilvenytė, Greta, Holubnycha, Viktoriia, Korniienko, Viktoriia, Diedkova, Kateryna, Viter, Roman, Ramanavičius, Arūnas |
| المصدر: |
Polymers., Basel : MDPI AG, 2023, vol. 15, iss. 7, art. no. 1597, p. 1-16. ; eISSN 2073-4360 |
| سنة النشر: |
2023 |
| المجموعة: |
Vilnius University Virtual Library (VU VL) / Vilniaus universitetas virtuali biblioteka |
| مصطلحات موضوعية: |
molecularly imprinted polymer, molecularly imprinted polypyrrole, Listeria monocytogenes, whole-cell imprinting, pulsed amperometric detection, template extraction method, trypsin, L-lysine, acetic acid, sulphuric acid |
| الوصف: |
Detecting bacteria—Listeria monocytogenes—is an essential healthcare and food industry issue. The objective of the current study was to apply platinum (Pt) and screen-printed carbon (SPCE) electrodes modified by molecularly imprinted polymer (MIP) in the design of an electrochemical sensor for the detection of Listeria monocytogenes. A sequence of potential pulses was used to perform the electrochemical deposition of the non-imprinted polypyrrole (NIP-Ppy) layer and Listeria monocytogenes-imprinted polypyrrole (MIP-Ppy) layer over SPCE and Pt electrodes. The bacteria were removed by incubating Ppy-modified electrodes in different extraction solutions (sulphuric acid, acetic acid, L-lysine, and trypsin) to determine the most efficient solution for extraction and to obtain a more sensitive and repeatable design of the sensor. The performance of MIP-Ppy- and NIP-Ppy-modified electrodes was evaluated by pulsed amperometric detection (PAD). According to the results of this research, it can be assumed that the most effective MIP-Ppy/SPCE sensor can be designed by removing bacteria with the proteolytic enzyme trypsin. The LOD and LOQ of the MIP-Ppy/SPCE were 70 CFU/mL and 210 CFU/mL, respectively, with a linear range from 300 to 6700 CFU/mL. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://epublications.vu.lt/object/elaba:162809982/162809982.pdf; https://repository.vu.lt/VU:ELABAPDB162809982&prefLang=en_US |
| الاتاحة: |
https://repository.vu.lt/VU:ELABAPDB162809982&prefLang=en_US |
| Rights: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.8F303BB5 |
| قاعدة البيانات: |
BASE |