Academic Journal
Activation of ErbB-2 via a hierarchical interaction between ErbB-2 and type I insalia-like growth factor receptor in mammary tumor cells
| العنوان: | Activation of ErbB-2 via a hierarchical interaction between ErbB-2 and type I insalia-like growth factor receptor in mammary tumor cells |
|---|---|
| المؤلفون: | Balãá, M.E., Labriola, L., Salatino, M., Movsichoff, F., Peters, G., Charreau, E.H., Elizalde, P.V. |
| المصدر: | Oncogene 2001;20(1):34-47 |
| سنة النشر: | 2001 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | ErbB receptors, Mammary tumorigenesis, Progestin, Type I insulin-like growth factor receptor, antisense oligodeoxynucleotide, gestagen, medroxyprogesterone acetate, messenger RNA, neu differentiation factor, oligomer, protein tyrosine kinase, somatomedin C receptor, animal cell, animal experiment, animal model, animal tissue, article, breast carcinogenesis, breast epithelium, breast tumor, cell proliferation, complex formation, confocal laser microscopy, controlled study, female, gene activation, mouse, nonhuman, oncogene neu, priority journal |
| الوصف: | The present study focused on interactions between signaling pathways activated by progestins and by type I and II receptor tyrosine kinases (RTKs) in mammary tumors. An experimental model in which the synthetic progestin medroxyprogesterone acetate (MPA) induced mammary adenocarcinomas in Balb/c mice was used. MPA-stimulated proliferation, both in vivo and in vitro, of progestin-dependent tumors induced up-regulation of ErbB-2 protein levels and tyrosine phosphorylation of this receptor. Combinations of antisense oligodeoxynueleotides (ASODNs) directed to ErbB-2 mRNA with ASODNs directed to the insulin-like growth factor-I receptor (IGF-IR) were used to study the effect of the simultaneous block of these receptors on the MPA-induced proliferation of epithelial cells from the progestin-dependent C4HD line. Neither synergistic nor additive effects on the inhibition of MPA-induced proliferation of C4HD cells were observed as a result of the combination of these ASODNs. Suppression of IGF-IR expression by ASODNs resulted in complete abrogation of MPA-induced phosphorylation of ErbB-2 in C4HD cells, whereas blockage of ErbB-2 did not affect IGF-IR phosphorylation. These results show the existence of a hierarchical interaction between IGF-IR and ErbB-2, by means of which IGF-IR directs ErbB-2 phosphorylation. We demonstrated, for the first time, that this hierarchical interaction involves physical association of both receptors, resulting in the formation of a heteromeric complex. Furthermore, confocal laser microscopy experiments demonstrated that MPA was able to induce co-localization of ErbB-2 and IGF-IR. This hetero-oligomer was also found in MCF-7 human breast cancer cells in which association of IGF-IR and ErbB-2 was induced by heregulin and IGF-I. ; Fil:Labriola, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Salatino, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Movsichoff, F. Universidad de Buenos Aires. Facultad de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | http://hdl.handle.net/20.500.12110/paper_09509232_v20_n1_p34_Balaa; http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09509232_v20_n1_p34_Balaa_oai |
| الاتاحة: | https://hdl.handle.net/20.500.12110/paper_09509232_v20_n1_p34_Balaa http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_09509232_v20_n1_p34_Balaa_oai |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/ar |
| رقم الانضمام: | edsbas.8F2AA35D |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |