Academic Journal
Trace levels of sewage effluent are sufficient to increase class 1 integron prevalence in freshwater biofilms without changing the core community.
| العنوان: | Trace levels of sewage effluent are sufficient to increase class 1 integron prevalence in freshwater biofilms without changing the core community. |
|---|---|
| المؤلفون: | Lehmann, K, Bell, T, Bowes, MJ, Amos, GCA, Gaze, WH, Wellington, EMH, Singer, AC |
| بيانات النشر: | IWA Publishing & Elsevier |
| سنة النشر: | 2016 |
| المجموعة: | University of Exeter: Open Research Exeter (ORE) |
| مصطلحات موضوعية: | Antibiotic resistance, Biofilms, Class 1 integron-integrase gene, River ecology, River health, Sewage effluent |
| الوصف: | This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record. ; Most river systems are impacted by sewage effluent. It remains unclear if there is a lower threshold to the concentration of sewage effluent that can significantly change the structure of the microbial community and its mobile genetic elements in a natural river biofilm. We used novel in situ mesocosms to conduct replicated experiments to study how the addition of low-level concentrations of sewage effluent (nominally 2.5 ppm) affects river biofilms in two contrasting Chalk river systems, the Rivers Kennet and Lambourn (high/low sewage impact, respectively). 16S sequencing and qPCR showed that community composition was not significantly changed by the sewage effluent addition, but class 1 integron prevalence (Lambourn control 0.07% (SE ± 0.01), Lambourn sewage effluent 0.11% (SE ± 0.006), Kennet control 0.56% (SE ± 0.01), Kennet sewage effluent 1.28% (SE ± 0.16)) was significantly greater in the communities exposed to sewage effluent than in the control flumes (ANOVA, F = 5.11, p = 0.045) in both rivers. Furthermore, the difference in integron prevalence between the Kennet control (no sewage effluent addition) and Kennet sewage-treated samples was proportionally greater than the difference in prevalence between the Lambourn control and sewage-treated samples (ANOVA (interaction between treatment and river), F = 6.42, p = 0.028). Mechanisms that lead to such differences could include macronutrient/biofilm or phage/bacteria interactions. Our findings highlight the role that low-level exposure to complex polluting mixtures such as sewage effluent can play in the spread of antibiotic resistance genes. The results also highlight that certain conditions, such as macronutrient load, might accelerate spread of antibiotic resistance genes. ; This work was funded by the NERC Centre for Ecology & Hydrology National Capability funding through the CEH Thames Initiative (NEC04877) and the NERCeKnowledge ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://www.ncbi.nlm.nih.gov/pubmed/27710799; http://hdl.handle.net/10871/25146; Water Research |
| DOI: | 10.1016/j.watres.2016.09.035 |
| الاتاحة: | http://hdl.handle.net/10871/25146 https://doi.org/10.1016/j.watres.2016.09.035 |
| Rights: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/) |
| رقم الانضمام: | edsbas.8EDCB9A1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.watres.2016.09.035 |
|---|