Academic Journal
Development of enhanced buccal films with Actinidia arguta fruit extract for oral mucositis prevention: from in vitro buccal models to ex vivo investigations
| العنوان: | Development of enhanced buccal films with Actinidia arguta fruit extract for oral mucositis prevention: from in vitro buccal models to ex vivo investigations |
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| المؤلفون: | Teixeira, Filipa, Silva, Ana Margarida, Macedo, Catarina, Estevinho, Berta, Sut, Stefania, Dall’Acqua, Stefano, Delerue-Matos, Cristina, Costa, Paulo C., Rodrigues, Francisca |
| المصدر: | Scientific Letters; Vol. 1 No. Sup 1 (2024) ; 2795-5117 |
| بيانات النشر: | IUCS-CESPU Publishing |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Selected Oral Communication |
| الوصف: | Background:Oral mucositis (OM) is a common side effect of cancer treatments such as chemotherapy [1], being characterized by disruption of the oral mucosa integrity, inflammation, and pain [1,2]. The treatment strategies to prevent and treat OM are still unsatisfactory, leading to the search of new active compounds, particularly from natural sources, such as Actinidia arguta fruits [1]. A. arguta fruit, commonly known as kiwiberry, has been associated with different therapeutic properties and pro-healthy benefits, particularly antioxidant, anti-inflammatory and anticancer effects [3,4], due to the fruit’s outstanding content in phenolic compounds, vitamins, and organic acids [3,4]. Objective: The aim of this study was to develop buccal films with A. arguta fruit extract as active ingredient to prevent OM symptoms. Methods: The films were prepared by solvent casting employing 1% of HPMC K100 LV EP, 2.5% glycerin, and A. arguta extract as solvent, previously prepared by Ultrasound-Assisted Extraction [4]. Results: Different films parameters were assessed, namely physical features (weight: 194.8 mg; thickness: 0.37 mm; disintegration time: 15.05 min; moisture content: 10.53%; swelling capacity: 55.95%), mechanical properties (resistance to extension: 10.11 N; percent of elongation: 36.10%; Young’s modulus: 0.0034 MPa) and antioxidant/antiradical activities (TPC = 6.46 mg GAE/g film; FRAP = 49.45 µmol FSE/g film; ABTS = 3.74 mg AAE/g film; DPPH = 4.90 mg TE/g film). In vitro cell assays attested the absence of negative effects on HSC-3 and TR146 oral cell lines. Most important, the compounds release profile was assessed through in vitro cell models (TR146) and ex vivo assay with porcine mucosa coupled to LC/DAD-ESI-MS quantification. The results revealed high permeation of rutin (88.32%), quercetin-3-O-glucoside (84.95%) and catechin (79.74%). Conclusions:Overall, these results highlight the significant potential and safety of buccal films with A. arguta fruit extract to prevent OM condition. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://publicacoes.cespu.pt/index.php/sl/article/view/139/150; https://publicacoes.cespu.pt/index.php/sl/article/view/139 |
| DOI: | 10.48797/sl.2024.139 |
| الاتاحة: | https://publicacoes.cespu.pt/index.php/sl/article/view/139 https://doi.org/10.48797/sl.2024.139 |
| Rights: | Copyright (c) 2024 Filipa Teixeira, Ana Margarida Silva, Catarina Macedo, Berta Estevinho, Stefania Sut, Stefano Dall’Acqua, Cristina Delerue-Matos, Paulo C. Costa, Francisca Rodrigues ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.8EAF8818 |
| قاعدة البيانات: | BASE |
| DOI: | 10.48797/sl.2024.139 |
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