Academic Journal
Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting
| العنوان: | Detection and quantification of SARS-CoV-2 in wastewater using a mobile laboratory setting |
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| المؤلفون: | Mathur, Shubham, Salah, Ikram, Msarwa, Haneen, Craddock, Hillary A, Moran-Gilad, Jacob |
| المساهمون: | Kando Environment Services Ltd |
| المصدر: | Letters in Applied Microbiology ; volume 78, issue 1 ; ISSN 1472-765X |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2024 |
| الوصف: | This study evaluated a point-of-care device for wastewater-based epidemiology (WBE) of SARS-CoV-2 circulation. WBE is a critical tool but faces challenges such as laboratory infrastructure, sample transport, skilled personnel, and time-consuming methods. Currently, a rapid, portable solution for wastewater surveillance is lacking. From June to September 2022, the performance of the GeneXpert instrument was compared with an established in-house method for detecting SARS-CoV-2 in 151 wastewater samples across laboratory, field, and mobile testing environments. In the laboratory setting, GeneXpert showed 100% agreement in detection, with 94.6% of positive samples displaying a viral load difference of ± 1 log compared to the in-house method. Field testing demonstrated 90% agreement, with 92.6% of GeneXpert-positive samples showing similar log differences. In mobile testing, GeneXpert detected 62.5% of samples, and testing faced technical issues. Overall, GeneXpert displayed a limit of detection of 12 cpml−1 with results available in under an hour, making it a promising tool for rapid and deployable wastewater testing. This requires further validation while addressing technical challenges related to field and mobile settings. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/lambio/ovae135 |
| DOI: | 10.1093/lambio/ovae135/61240464/ovae135.pdf |
| الاتاحة: | https://doi.org/10.1093/lambio/ovae135 https://academic.oup.com/lambio/advance-article-pdf/doi/10.1093/lambio/ovae135/61240464/ovae135.pdf https://academic.oup.com/lambio/article-pdf/78/1/ovae135/61240464/ovae135.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.8E29956D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/lambio/ovae135 |
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