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A novel technique for selective NF-{kappa}B inhibition in Kupffer cells: contrary effects in fulminant hepatitis and ischaemia-reperfusion

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العنوان: A novel technique for selective NF-{kappa}B inhibition in Kupffer cells: contrary effects in fulminant hepatitis and ischaemia-reperfusion
المؤلفون: Hoffmann, F, Sass, G, Zillies, J, Zahler, S, Tiegs, G, Hartkorn, A, Fuchs, S, Wagner, J, Winter, G, Coester, C, Gerbes, A L, Vollmar, A M
بيانات النشر: BMJ Publishing Group Ltd
سنة النشر: 2009
المجموعة: HighWire Press (Stanford University)
مصطلحات موضوعية: Hepatology
الوصف: Background and aims: The transcription factor nuclear factor kappa B (NF-κB) has risen as a promising target for anti-inflammatory therapeutics. In the liver, however, NF-κB inhibition mediates both damaging and protective effects. The outcome is deemed to depend on the liver cell type addressed. Recent gene knock-out studies focused on the role of NF-κB in hepatocytes, whereas the role of NF-κB in Kupffer cells has not yet been investigated in vivo. Here we present a novel approach, which may be suitable for clinical application, to selectively target NF-κB in Kupffer cells and analyse the effects in experimental models of liver injury. Methods: NF-κB inhibiting decoy oligodeoxynucleotides were loaded upon gelatin nanoparticles (D-NPs) and their in vivo distribution was determined by confocal microscopy. Liver damage, NF-κB activity, cytokine levels and apoptotic protein expression were evaluated after lipopolysaccharide (LPS), d-galactosamine (GalN)/LPS, or concanavalin A (ConA) challenge and partial warm ischaemia and subsequent reperfusion, respectively. Results: D-NPs were selectively taken up by Kupffer cells and inhibited NF-κB activation. Inhibition of NF-κB in Kupffer cells improved survival and reduced liver injury after GalN/LPS as well as after ConA challenge. While anti-apoptotic protein expression in liver tissue was not reduced, pro-apoptotic players such as cJun N-terminal kinase (JNK) were inhibited. In contrast, selective inhibition of NF-κB augmented reperfusion injury. Conclusions: NF-κB inhibiting decoy oligodeoxynucleotide-loaded gelatin nanoparticles is a novel tool to selectively inhibit NF-κB activation in Kupffer cells in vivo. Thus, liver injury can be reduced in experimental fulminant hepatitis, but increased at ischaemia–reperfusion.
نوع الوثيقة: text
وصف الملف: text/html
اللغة: English
Relation: http://gut.bmj.com/cgi/content/short/58/12/1670; http://dx.doi.org/10.1136/gut.2008.165647
DOI: 10.1136/gut.2008.165647
الاتاحة: http://gut.bmj.com/cgi/content/short/58/12/1670
https://doi.org/10.1136/gut.2008.165647
Rights: Copyright (C) 2009, BMJ Publishing Group
رقم الانضمام: edsbas.8D32EF9D
قاعدة البيانات: BASE
الوصف
DOI:10.1136/gut.2008.165647