التفاصيل البيبلوغرافية
| العنوان: |
Nitrate-Driven Trophic Association of Sulfur-Cycling Microorganisms in Tsunami-Deposited Marine Sediment Revealed by High-Sensitivity 13 C‑Bicarbonate Probing |
| المؤلفون: |
Tomo Aoyagi (1572826), Yoko Katayama (1982467), Hidenobu Aizawa (440985), Mitsuru Takasaki (10904559), Tomoyuki Hori (1572823) |
| سنة النشر: |
1753 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Ecology, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Desulfofustis glycolicus relatives, syntrophic volatile-fatty-acid oxidizer, activation, marine ecosystem function, Tsunami-Deposited Marine Sediment R., denitrification-dependent chemolith., latter nutrient-limited conditions, i.e, denitrification-dependent sulfur-ox., nitrate-amended sediment microorganisms, 13 CO 2, nitrate-driven trophic association, sulfur-cycling microorganisms, tsunami-deposited marine sediment, biogeochemical carbon transfer, hydrolytic Pararheinheimera aquatica, 13 C, 16 S rRNA molecules, fermentative Thalassomonas sediminis, HDS 22 relatives, sp, sulfur oxidation co-occurred, time-resolved high-sensitivity 13 C. |
| الوصف: |
Although denitrification-dependent chemolithotrophic sulfur oxidizers proliferated in tsunami-deposited marine sediment with nitrate amendment, their ecophysiological roles in biogeochemical carbon transfer are not addressed. We employed time-resolved high-sensitivity 13 C-bicarbonate probing of rRNA to unveil the carbon fixation and resulting trophic relationship of the nitrate-amended sediment microorganisms. Nitrate reduction and sulfur oxidation co-occurred along with significant decreases in the 13 CO 2 and dissolved bicarbonate concentrations for the first 4 days of the incubation, during which the denitrification-dependent sulfur-oxidizing chemolithotrophs, i.e., the Sulfurimonas sp. HDS01 and Thioalkalispira sp. HDS22 relatives, and the sulfate-reducing heterotrophs, i.e., the Desulfobulbus spp. and Desulfofustis glycolicus relatives, actively incorporated 13 C. These indicated that the sulfur oxidizers and sulfate reducers were tightly associated with each other through the direct carbon transfer. Relatives of the fermentative Thalassomonas sediminis and the hydrolytic Pararheinheimera aquatica , in addition to various sulfur-cycling microorganisms, significantly assimilated 13 C at day 14. Although the incorporation of 13 C was not detected, a syntrophic volatile-fatty-acid oxidizer and hydrogenotrophic methanogens significantly expressed their 16S rRNA molecules at day 21, indicating the metabolic activation of these final decomposers under the latter nutrient-limited conditions. The results demonstrated the nitrate-driven trophic association of sulfur-cycling microorganisms and the subsequent microbial activation and diversification, triggering the restoration of the marine ecosystem function. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Nitrate-Driven_Trophic_Association_of_Sulfur-Cycling_Microorganisms_in_Tsunami-Deposited_Marine_Sediment_Revealed_by_High-Sensitivity_sup_13_sup_C_Bicarbonate_Probing/14710716 |
| DOI: |
10.1021/acs.est.0c08191.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.est.0c08191.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.8D0D3DAC |
| قاعدة البيانات: |
BASE |