Academic Journal
A new mouse-adapted strain of SARS-CoV as a lethal model forevaluating antiviral agents in vitro and in vivo
| العنوان: | A new mouse-adapted strain of SARS-CoV as a lethal model forevaluating antiviral agents in vitro and in vivo |
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| المؤلفون: | Day, C. W., Baric, R., Cai, S. X., Frieman, M., Kumaki, Y., Morrey, John D, Smee, Donald F., Barnard, Dale L. |
| المصدر: | Animal, Dairy, and Veterinary Science Faculty Publications |
| بيانات النشر: | Hosted by Utah State University Libraries |
| سنة النشر: | 2009 |
| المجموعة: | Utah State University: DigitalCommons@USU |
| مصطلحات موضوعية: | SARS, antiviral, vivo, adapted strain, Animal Sciences, Dairy Science |
| الوصف: | Severe acute respiratory syndrome (SARS) is a highly lethal emerging disease caused by coronavirus SARS-CoV. New lethal animal models for SARS were needed to facilitate antiviral research. We adapted and characterized a new strain of SARS-CoV (strain v2163) that was highly lethal in 5–6 week old BALB/c mice. It had nine mutations affecting 10 amino acid residues. Strain v2163 increased IL-1α, IL-6, MIP-1α, MCP-1, and RANTES in mice, and high IL-6 expression correlated with mortality. The infection largely mimicked human disease, but lung pathology lacked hyaline membrane formation. In vitro efficacy against v2163 was shown with known inhihibitors of SARSCoV replication. In v2163-infected mice, Ampligen™ was fully protective, stinging nettle lectin (UDA) was partially protective, ribavirin was disputable and possibly exacerbated disease, and EP128533 was inactive. Ribavirin, UDA and Ampligen™ decreased IL-6 expression. Strain v2163 provided a valuable model for anti-SARS research. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| Relation: | https://digitalcommons.usu.edu/advs_facpub/528; https://digitalcommons.usu.edu/context/advs_facpub/article/1527/viewcontent/A_new_mouse_adapted_strain_of_SARS_CoV_as_a_lethal_model_for_evaluating_antiviral_agents_in_vitro_and_in_vivo.pdf |
| DOI: | 10.1016/j.virol.2009.09.023 |
| الاتاحة: | https://digitalcommons.usu.edu/advs_facpub/528 https://doi.org/10.1016/j.virol.2009.09.023 https://digitalcommons.usu.edu/context/advs_facpub/article/1527/viewcontent/A_new_mouse_adapted_strain_of_SARS_CoV_as_a_lethal_model_for_evaluating_antiviral_agents_in_vitro_and_in_vivo.pdf |
| Rights: | Copyright for this work is held by the author. Transmission or reproduction of materials protected by copyright beyond that allowed by fair use requires the written permission of the copyright owners. Works not in the public domain cannot be commercially exploited without permission of the copyright owner. Responsibility for any use rests exclusively with the user. For more information contact the Institutional Repository Librarian at digitalcommons@usu.edu. |
| رقم الانضمام: | edsbas.8CE483A1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.virol.2009.09.023 |
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