Academic Journal
A novel role for Gtb1p in glucose trimming of N-linked glycans
| العنوان: | A novel role for Gtb1p in glucose trimming of N-linked glycans |
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| المؤلفون: | Quinn, Robert P, Mahoney, Sarah J, Wilkinson, Barrie M, Thornton, David J, Stirling, Colin J |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2009 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | Original Article |
| الوصف: | Glucosidase II (GluII) is a glycan-trimming enzyme active on nascent glycoproteins in the endoplasmic reticulum (ER). It trims the middle and innermost glucose residues (Glc2 and Glc1) from N -linked glycans. The monoglucosylated glycan produced by the first GluII trimming reaction is recognized by calnexin/calreticulin and serves as the signal for entry into this folding pathway. GluII is a heterodimer of α and β subunits corresponding to yeast Gls2p and Gtb1p, respectively. While Gls2p contains the glucosyl hydrolase active site, the Gtb1p subunit has previously been shown to be essential for the Glc1 trimming event. Here we demonstrate that Gtb1p also determines the rate of Glc2 trimming. In order to further dissect these activities we mutagenized a number of conserved residues across the protein. Our data demonstrate that both the MRH and G2B domains of Gtb1p contribute to the Glc2 trimming event but that the MRH domain is essential for Glc1 trimming. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://glycob.oxfordjournals.org/cgi/content/short/19/12/1408; http://dx.doi.org/10.1093/glycob/cwp087 |
| DOI: | 10.1093/glycob/cwp087 |
| الاتاحة: | http://glycob.oxfordjournals.org/cgi/content/short/19/12/1408 https://doi.org/10.1093/glycob/cwp087 |
| Rights: | Copyright (C) 2009, Society for Glycobiology |
| رقم الانضمام: | edsbas.8C1F26A8 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/glycob/cwp087 |
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