التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets |
| المؤلفون: |
JUAN DIEGO CORTES GARCIA, CINTYA DEL REFUGIO LOPEZ LOPEZ, NANCY CORTEZ ESPINOSA, MARIANA HAYDEE GARCIA HERNANDEZ, JUAN MANUEL GUZMAN FLORES, ESTHER LAYSECA ESPINOSA, LILIANA PORTALES CERVANTES, DIANA PATRICIA PORTALES PEREZ |
| بيانات النشر: |
Elsevier |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
info:eu-repo/classification/Autor/P2X7 receptor, info:eu-repo/classification/Autor/ART1, info:eu-repo/classification/Autor/ATP, info:eu-repo/classification/Autor/NAD, info:eu-repo/classification/Autor/Immune regulation, info:eu-repo/classification/Autor/CD39, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2415 |
| الوصف: |
"Regulatory T cells that express CD39 (CD39+ Treg) exhibit specific immunomodulatory properties. Ectonucleotidase CD39 hydrolyses ATP and ADP. ATP is a ligand of the P2X7 receptor and induces the shedding of CD62L and apoptosis. However, the role of ATP in CD39+ Treg cells has not been defined. Furthermore, NAD can activate the P2X7 receptor via ADP-ribosyltransferase (ART) enzymes and cause cell depletion in murine models. We evaluated the expression and function of P2X7 and ART1 in CD39+ Treg and CD39- Treg cells in the presence or absence of ATP and NAD. We isolated peripheral blood mononuclear cells from healthy subjects and purified CD4+ T cells, CD4+ CD25+ T cells and CD4+ CD25+ CD39+ T cells. P2X7 and ART1 expression was assessed by flow cytometry and real-time PCR. Our results showed low P2X7 expression on CD39+ Treg cells and higher levels of ART1 expression in CD4+ CD39+ T cells than the other subtypes studied. Neither shedding of CD62L nor cell death of CD39+ Treg or CD39- Treg cells was observed by 1mM ATP or 60 mu M NAD. In contrast, P2Xs receptor-dependent proliferation with 300 p,M ATP, was inhibited by NAD in the different cell types analysed. The NAD proliferation-inhibition was increased with P2X5 and A2a agonist and was reversed with P2X5 and A2a antagonist, therefore NAD inhibits P2Xs-dependent proliferation and A2a activation. In conclusion, our results suggest that the altered function and expression of P2X7 and ART1 in the human CD39+ Treg or CD39 Treg cells could participate in the resistance against cell death induced by ATP or NAD. (C) 2015 Elsevier GmbH. All rights reserved." |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
unknown |
| Relation: |
citation:Juan D. Cortés-Garcia, Cintya López-López, Nancy Cortez-Espinosa, Mariana H. García-Hernández, Juan M. Guzmán-Flores, Esther Layseca-Espinosa, Liliana Portales-Cervantes, Diana P. Portales-Pérez, Evaluation of the expression and function of the P2X7 receptor and ART1 in human regulatory T-cell subsets, Immunobiology, Volume 221, Issue 1, 2016, Pages 84-93.; http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1945 |
| الاتاحة: |
http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1945 |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0 |
| رقم الانضمام: |
edsbas.8C0DE4C4 |
| قاعدة البيانات: |
BASE |