Academic Journal
The branching ratio of enzymatically synthesized α-glucans impacts microbiome and metabolic outcomes of in vitro fecal fermentation
| العنوان: | The branching ratio of enzymatically synthesized α-glucans impacts microbiome and metabolic outcomes of in vitro fecal fermentation |
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| المؤلفون: | Deemer, Dane, Sarıoğlan, İhsan, Yaşar, Arife, Tunçil, Yunus E., Lee, Byung-Hoo, Lindemann, Stephen R., Yoo, Sang-Ho, ÇETİN, Bülent, Esen, Emine, Ryu, Hye-Jung |
| سنة النشر: | 2024 |
| الوصف: | The aim of this study was to evaluate the impacts of enzymatically synthesized α-glucans possessing α-1,4- and α-1,6-glucose linkages, and varying in branching ratio, on colonic microbiota composition and metabolic function. Four different α-glucans varying in branching ratio were synthesized by amylosucrase from Neisseria polysaccharea and glycogen branching enzyme from Rhodothermus obamensis. The branching ratios were found to range from 0 % to 2.8 % using GC/MS. In vitro fecal fermentation analyses (n = 8) revealed that the branching ratio dictates the short-chain fatty acid (SCFA) generation by fecal microbiota. Specifically, slightly branched (0.49 %) α-glucan resulted in generation of significantly (P < 0.05) higher amounts of propionate, compared to more-branched counterparts. In addition, the amount of butyrate generated from this α-glucan was statistically (P > 0.05) indistinguishable than those observed in resistant starches. 16S rRNA sequencing revealed that enzymatically synthesized α-glucans stimulated Lachnospiraceae and Ruminococcus related OTUs. Overall, the results demonstrated metabolic function of colonic microbiota can be manipulated by altering the branching ratio of enzymatically synthesized α-glucans, providing insights into specific structure-function relationships between dietary fibers and the colonic microbiome. Furthermore, the slightly branched α-glucans could be used as functional carbohydrates to stimulate the beneficial microbiota and SCFAs in the colon. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | 62042578-7a19-4609-aeb1-83bf302dcbff; https://avesis.atauni.edu.tr/publication/details/62042578-7a19-4609-aeb1-83bf302dcbff/oai |
| DOI: | 10.1016/j.carbpol.2024.122087 |
| الاتاحة: | https://doi.org/10.1016/j.carbpol.2024.122087 https://avesis.atauni.edu.tr/publication/details/62042578-7a19-4609-aeb1-83bf302dcbff/oai |
| Rights: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.8BF83458 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.carbpol.2024.122087 |
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