Academic Journal
Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress
| العنوان: | Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress |
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| المؤلفون: | Rupel, Katia, Zupin, Luisa, Ottaviani, Giulia, Bertani, Iris, Martinelli, Valentina, Porrelli, Davide, Vodret, Simone, Vuerich, Roman, Passos da Silva, Daniel, Bussani, Rossana, Crovella, Sergio, Parsek, Matthew, Venturi, Vittorio, Di Lenarda, Roberto, Biasotto, Matteo, Zacchigna, Serena |
| المساهمون: | International Centre for Genetic Engineering and Biotechnology |
| المصدر: | npj Biofilms and Microbiomes ; volume 5, issue 1 ; ISSN 2055-5008 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2019 |
| الوصف: | Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41522-019-0102-9 |
| الاتاحة: | http://dx.doi.org/10.1038/s41522-019-0102-9 https://www.nature.com/articles/s41522-019-0102-9.pdf https://www.nature.com/articles/s41522-019-0102-9 |
| Rights: | https://creativecommons.org/licenses/by/4.0 ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: | edsbas.8BEDC1C9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41522-019-0102-9 |
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