Academic Journal
A distinct class of slow (~0.2–2 Hz)) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex
| العنوان: | A distinct class of slow (~0.2–2 Hz)) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex |
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| المؤلفون: | Lorincz, Magor L., Gunner, David, Bao, Ying, Connelly, William M., Isaac, John T. R., Hughes, Stuart W., Crunelli, Vincenzo |
| بيانات النشر: | Society for Neuroscience |
| سنة النشر: | 2015 |
| المجموعة: | Cardiff University: ORCA (Online Research @ Cardiff) |
| مصطلحات موضوعية: | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| الوصف: | During sleep and anesthesia, neocortical neurons exhibit rhythmic UP/DOWN membrane potential states. Although UP states are maintained by synaptic activity, the mechanisms that underlie the initiation and robust rhythmicity of UP states are unknown. Using a physiologically validated model of UP/DOWN state generation in mouse neocortical slices whereby the cholinergic tone present in vivo is reinstated, we show that the regular initiation of UP states is driven by an electrophysiologically distinct subset of morphologically identified layer 5 neurons, which exhibit intrinsic rhythmic low-frequency burst firing at 0.2–2 Hz. This low-frequency bursting is resistant to block of glutamatergic and GABAergic transmission but is absent when slices are maintained in a low Ca 2 medium (an alternative, widely used model of cortical UP/DOWN states), thus explaining the lack of rhythmic UP states and abnormally prolonged DOWN states in this condition. We also characterized the activity of various other pyramidal and nonpyramidal neurons during UP/ DOWN states andfoundthat an electrophysiologically distinct subset of layer 5 regular spiking pyramidal neuronsfires earlier duringthe onset of network oscillations compared with all other types of neurons recorded. This study, therefore, identifies an important role for cell-type-specific neuronal activity in driving neocortical UP states. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://orca.cardiff.ac.uk/id/eprint/73654/1/lorincz%20et%20al%202015.pdf; Lorincz, Magor L., Gunner, David, Bao, Ying, Connelly, William M., Isaac, John T. R., Hughes, Stuart W. and Crunelli, Vincenzo https://orca.cardiff.ac.uk/view/cardiffauthors/A032685F.html orcid:0000-0001-7154-9752 orcid:0000-0001-7154-9752 2015. A distinct class of slow (~0.2–2 Hz)) intrinsically bursting layer 5 pyramidal neurons determines UP/DOWN state dynamics in the neocortex. Journal of Neuroscience 35 (14) , pp. 5442-5458. 10.1523/JNEUROSCI.3603-14.2015 https://doi.org/10.1523/JNEUROSCI.3603-14.2015 file https://orca.cardiff.ac.uk/id/eprint/73654/1/lorincz%20et%20al%202015.pdf |
| DOI: | 10.1523/JNEUROSCI.3603-14.2015 |
| الاتاحة: | https://orca.cardiff.ac.uk/id/eprint/73654/ https://doi.org/10.1523/JNEUROSCI.3603-14.2015 https://orca.cardiff.ac.uk/id/eprint/73654/1/lorincz%20et%20al%202015.pdf |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.8B787B4A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1523/JNEUROSCI.3603-14.2015 |
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